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Product Info Summary
| SKU: | A00235-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, WB, IHC-P-Leica |
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Product info
Product Name
Anti-Vimentin Antibody
SKU/Catalog Number
A00235-1
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-Vimentin Antibody (Catalog # A00235-1). Tested in WB, IF, Flow Cytometry, IHC-P-Leica application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-Vimentin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00235-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This Vimentin antibody is generated from rabbits immunized with human Vimentin recombinant protein.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00235-1 is reactive to VIM in Human, Mouse
Calculated molecular weight
53.7 kDa
Background of VIM
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00235-1 is guaranteed for Flow Cytometry, IF, WB, IHC-P-Leica Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:50
WB: 1:2000
IHC-P-Leica: 1:500
FC: 1:50
Validation Images & Assay Conditions
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Confocal immunofluorescent analysis of Vimentin Antibody with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
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Confocal immunofluorescent analysis of Vimentin Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
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Confocal immunofluorescent analysis of Vimentin Antibody with U251 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
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All lanes : Anti-VIME Antibody at 1:4000 dilution
Lane 1: Hela whole cell lysate
Lane 2: 293 whole cell lysate
Lane 3: U-2OS whole cell lysate
Lane 4: Jurkat whole cell lysate
Lane 5: K562 whole cell lysate
Lane 6: NIH/3T3 whole cell lysate
Lane 7: C2C12 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 54 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-VIME Antibody at 1:2000 dilution
Lane 1: Hela whole cell lysate
Lane 2: 293 whole cell lysate
Lane 3: U-2OS whole cell lysate
Lane 4: Jurkat whole cell lysate
Lane 5: K562 whole cell lysate
Lane 6: NIH/3T3 whole cell lysate
Lane 7: C2C12 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 54 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded Human small intestine tissue using A00235-1 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
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Immunohistochemical analysis of paraffin-embedded Human small intestine tissue using A00235-1 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Specific Publications For Anti-Vimentin Antibody (A00235-1)
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