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- Table of Contents
VIM antibody (A00235-1) performs well in Flow Cytometry, IF, WB, IHC-P-Leica and shows reactivity with Human, Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Confocal immunofluorescent analysis of Vimentin Antibody with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
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Anti-Vimentin Antibody
VIM antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse | Rabbit | Flow Cytometry, IF, WB, IHC-P-Leica |
Recommend Dilution
Application
Dilution
Species
IF: 1:50
WB: 1:2000
IHC-P-Leica: 1:500
FC: 1:50
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Vimentin Antibody
View all VIM Antibodies
View all VIM Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This Vimentin antibody is generated from rabbits immunized with human Vimentin recombinant protein.
Cross-Reactivity
No cross reactivity with other proteins.
Calculated Molecular Weight
53.7 kDa
Background of VIM
This gene encodes a member of the intermediate filament
family. Intermediate filamentents, along with microtubules and
actin microfilaments, make up the cytoskeleton. The protein encoded
by this gene is responsible for maintaining cell shape, integrity
of the cytoplasm, and stabilizing cytoskeletal interactions. It is
also involved in the immune response, and controls the transport of
low-density lipoprotein (LDL)-derived cholesterol from a lysosome
to the site of esterification. It functions as an organizer of a
number of critical proteins involved in attachment, migration, and
cell signaling. Mutations in this gene causes a dominant,
pulverulent cataract.
Cite This Product
Anti-Vimentin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00235-1)
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We promise Anti-Vimentin Antibody performs excellently in relevant applications for Flow Cytometry, IF, WB, IHC-P-Leica. If it doesn't perform as promised, we will refund the product. Learn more
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A00235-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Sep 22, 2026