This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
RBM27 antibody (A13137) performs well in ELISA, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
Filter:
IHC analysis of RBM27 using anti-RBM27 antibody (A13137).
RBM27 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 200 rabbit anti-RBM27 Antibody (A13137) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
RBM27 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 200 rabbit anti-RBM27 Antibody (A13137) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Bundle Save
Anti-RBM27 Antibody
RBM27 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IF, IHC, ICC, WB |
Tested Applications
RBM27 antibody was positively detected by WB in:
human Hela whole cell, human K562 whole cell, human SIHA whole cell, human HEL whole cell, human Caco-2 whole cell, human A431 whole cell, human A549 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
RBM27 antibody was positively detected by IHC in:
human breast cancer tissue, human lung cancer tissue, mouse testis tissue, rat testis tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
1:500-2000
Immunohistochemistry
1:50-400
Immunocytochemistry/Immunofluorescence
1:50-400
ELISA
1:100-1000
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
IHC
ICC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Recommend
Rat
Tested
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-RBM27 Antibody
View all RBM27 Antibodies
View all RBM27 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
500 μg/ml antibody with PBS, 0.02% NaN3, 1 mg stabilizing protein and 50% glycerol
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Immunogen
E.coli-derived human RBM27 recombinant protein (Position: Q682-V812).
Observed Molecular Weight
140 kDa
Calculated Molecular Weight
118.7 kDa
Background of RBM27
RBM27 has been identified as a candidate autism gene, and is present in cytoplasmic and and nuclear speckles. RBM27 encodes a protein containing an RNA-Binding Motif, which confers its ability to bind RNA. Therefore, RBM27 is important in mRNA processing and the turnover of nuclear polyadenylate (pA+) RNA.
Cite This Product
Anti-RBM27 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13137)
Protocols
Western Blot protocol for RBM27 antibody
Immunohistochemistry (IHC) protocol for RBM27 antibody
Review
Have you used Anti-RBM27 Antibody?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-RBM27 Antibody performs excellently in relevant applications for ELISA, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A13137
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 4, 2026