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Product Info Summary
| SKU: | A14311-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-RBM41 Antibody Picoband®
SKU/Catalog Number
A14311-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-RBM41 Antibody Picoband® catalog # A14311-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-RBM41 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14311-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human RBM41 recombinant protein (Position: E130-F384).
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A14311-1 is reactive to RBM41 in Human, Mouse, Rat
Observed Molecular Weight
47 kDa
Calculated molecular weight
47.1 kDa
Background of RBM41
RBM41 is an RNA-binding protein of unknown specific function. The human gene is mapped to Xq22.3.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14311-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human SGC-7901 whole cell, human A549 whole cell, human HEK293 whole cell, rat liver tissue, mouse liver tissue, mouse HEPA1-6 whole cell
FCM: Jurkat cell
Validation Images & Assay Conditions
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Western blot analysis of RBM41 using anti-RBM41 antibody (A14311-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SGC-7901 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human HEK293 whole cell lysates,
Lane 4: rat liver tissue lysates,
Lane 5: mouse liver tissue lysates,
Lane 6: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBM41 antigen affinity purified polyclonal antibody (Catalog # A14311-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RBM41 at approximately 47 kDa. The expected band size for RBM41 is at 47 kDa.
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Flow Cytometry analysis of Jurkat cells using anti-RBM41 antibody (A14311-1).
Overlay histogram showing Jurkat cells stained with A14311-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RBM41 Antibody (A14311-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-RBM41 Antibody Picoband® (A14311-1)
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1 Customer Q&As for Anti-RBM41 Antibody Picoband®
Question
Is the antigen for A14311-1 a synthetic peptide?
Verified customer
Asked: 2021-11-01
Answer
The antigen for Anti-RBM41 Antibody Picoband™ (A14311-1) is a recombinant protein rather than a synthetic peptide.
Boster Scientific Support
Answered: 2021-11-02
