Anti-RBM5/Luca15 Antibody

RBM5 antibody

Boster Bio Anti-RBM5/Luca15 Antibody (Catalog # A03559). Tested in WB, IHC applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A03559
Size: 100 µl
Reactive Species: Human, Mouse
Host: Rabbit
Application: IHC, WB

Product Name

Anti-RBM5/Luca15 Antibody

View all RBM5 Antibodies

SKU/Catalog Number

A03559

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-RBM5/Luca15 Antibody (Catalog # A03559). Tested in WB, IHC applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-RBM5/Luca15 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03559)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human RBM5.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A03559 is reactive to RBM5 in Human, Mouse

Reconstitution

Calculated molecular weight

92154 MW

Background of RBM5

Component of the spliceosome A complex. Regulates alternative splicing of a number of mRNAs. May modulate splice site pairing after recruitment of the U1 and U2 snRNPs to the 5' and 3' splice sites of the intron. May both positively and negatively regulate aopotosis by regulating the alternative splicing of several genes involved in this process, including FAS and CASP2/caspase-2. In the case of FAS, promotes exclusion of exon 6 thereby producing a soluble form of FAS that inhibits apoptosis. In the case of CASP2/caspase-2, promotes exclusion of exon 9 thereby producing a catalytically active form of CASP2/Caspase-2 that induces apoptosis.

Bader S., Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases.
Timmer T., Genomics 60:238-240(1999).
Oh J.J., Nucleic Acids Res. 27:4008-4017(1999).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A03559 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Validation Images & Assay Conditions

Gene/Protein Information For RBM5 (Source: Uniprot.org, NCBI)

Gene Name

RBM5

Full Name

RNA-binding protein 5

Weight

92154 MW

Superfamily

RBM5/RBM10 family

Alternative Names

RNA-binding protein 5;Protein G15;Putative tumor suppressor LUCA15;RNA-binding motif protein 5;Renal carcinoma antigen NY-REN-9;RBM5;H37, LUCA15; RBM5 G15, H37, LUCA-15, LUCA15, RMB5 RNA binding motif protein 5 RNA-binding protein 5|putative tumor suppressor LUCA15|renal carcinoma NY-REN-9

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on RBM5, check out the RBM5 Infographic

RBM5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for RBM5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-RBM5/Luca15 Antibody

Question

Does A03559 anti-RBM5/Luca15 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-02-28

Answer

As indicated on the product datasheet, A03559 anti-RBM5/Luca15 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-02-28

Question

I was wanting to use your anti-RBM5/Luca15 antibody for WB for mouse testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse testis identification?

Verified Customer

Verified customer

Asked: 2020-02-14

Answer

As indicated on the product datasheet, A03559 anti-RBM5/Luca15 antibody has been validated for IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse testis in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-02-14

Question

Is a blocking peptide available for product anti-RBM5/Luca15 antibody (A03559)?

Verified Customer

Verified customer

Asked: 2019-12-30

Answer

We do provide the blocking peptide for product anti-RBM5/Luca15 antibody (A03559). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-12-30

Question

See attached the WB image, lot number and protocol we used for testis using anti-RBM5/Luca15 antibody A03559. Please let me know if you require anything else.

B. Johnson

Verified customer

Asked: 2019-11-20

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-20

Question

I am interested in to test anti-RBM5/Luca15 antibody A03559 on mouse testis for research purposes, then I may be interested in using anti-RBM5/Luca15 antibody A03559 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-09-03

Answer

The products we sell, including anti-RBM5/Luca15 antibody A03559, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-09-03

Question

Would anti-RBM5/Luca15 antibody A03559 work for WB with testis?

Verified Customer

Verified customer

Asked: 2019-06-28

Answer

According to the expression profile of testis, RBM5 is highly expressed in testis. So, it is likely that anti-RBM5/Luca15 antibody A03559 will work for WB with testis.

Boster Scientific Support

Answered: 2019-06-28

Question

We are currently using anti-RBM5/Luca15 antibody A03559 for mouse tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2019-06-03

Answer

The anti-RBM5/Luca15 antibody (A03559) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-06-03

Order DetailsPrice
A03559-100uL

100uL

$360

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A03559
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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