Anti-RBM5/Luca15 Antibody

Product Name

Anti-RBM5/Luca15 Antibody

View all RBM5 Antibodies

SKU/Catalog Number

A03559

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-RBM5/Luca15 Antibody (Catalog # A03559). Tested in WB, IHC applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-RBM5/Luca15 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03559)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human RBM5.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A03559 is reactive to RBM5 in Human, Mouse

Applications

A03559 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

92.154kDa

Background of RBM5

Component of the spliceosome A complex. Regulates alternative splicing of a number of mRNAs. May modulate splice site pairing after recruitment of the U1 and U2 snRNPs to the 5' and 3' splice sites of the intron. May both positively and negatively regulate aopotosis by regulating the alternative splicing of several genes involved in this process, including FAS and CASP2/caspase-2. In the case of FAS, promotes exclusion of exon 6 thereby producing a soluble form of FAS that inhibits apoptosis. In the case of CASP2/caspase-2, promotes exclusion of exon 9 thereby producing a catalytically active form of CASP2/Caspase-2 that induces apoptosis.

Bader S., Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases.
Timmer T., Genomics 60:238-240(1999).
Oh J.J., Nucleic Acids Res. 27:4008-4017(1999).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Validation Images & Assay Conditions

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Western blot analysis of extracts from HUVEC cells, using RBM5 antibody A03559.

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Immunohistochemistry analysis of paraffin-embedded human brain tissue using RBM5 antibody A03559.

Gene/Protein Information For RBM5 (Source: Uniprot.org, NCBI)

Alternative Names

G15; H37; LUCA15FLJ39876; Protein G15; Putative tumor suppressor LUCA15; Renal carcinoma antigen NY-REN-9; RMB5; RNA binding motif protein 5; RNA-binding motif protein 5; RNA-binding protein 5 RBM5 G15, H37, LUCA-15, LUCA15, RMB5 RNA binding motif protein 5 RNA-binding protein 5|putative tumor suppressor LUCA15|renal carcinoma antigen NY-REN-9

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In this infographic, you will see the following information for RBM5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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