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Product Info Summary
| SKU: | A06728-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-RBMY1A1 Antibody Picoband®
SKU/Catalog Number
A06728-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-RBMY1A1 Antibody Picoband® catalog # A06728-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-RBMY1A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06728-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human RBMY1A1 recombinant protein (Position: R188-Y496).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06728-1 is reactive to RBMY1A1 in Human, Mouse, Rat
Observed Molecular Weight
51 kDa
Calculated molecular weight
55.8 kDa
Background of RBMY1A1
RNA-binding motif protein, Y chromosome, family 1 member A1/C is a protein that in humans is encoded by the RBMY1A1 gene. This gene encodes a protein containing an RNA-binding motif in the N-terminus and four SRGY (serine, arginine, glycine, tyrosine) boxes in the C-terminus. This protein is thought to function as a splicing regulator during spermatogenesis. Multiple closely related paralogs of this gene are found in a gene cluster in the AZFb azoospermia factor region of chromosome Y. Most of these related copies are thought to be pseudogenes, though several likely encode functional proteins.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06728-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human HEL whole cell, rat brain tissue, mouse testis tissue, mouse brain tissue, mouse NIH/3T3 whole cell
ICC/IF: Hela cell
Validation Images & Assay Conditions
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Western blot analysis of RBMY1A1 using anti-RBMY1A1 antibody (A06728-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse testis tissue lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBMY1A1 antigen affinity purified polyclonal antibody (Catalog # A06728-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RBMY1A1 at approximately 51 kDa. The expected band size for RBMY1A1 is at 56 kDa.
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IF analysis of RBMY1A1 and Tubulin beta using anti-RBMY1A1 antibody (A06728-1) and anti-Tubulin beta antibody (M05613-4).
RBMY1A1 and Tubulin beta was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RBMY1A1 Antibody (A06728-1) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Specific Publications For Anti-RBMY1A1 Antibody Picoband® (A06728-1)
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