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Product Info Summary
| SKU: | A12027 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-RCL1 Antibody
SKU/Catalog Number
A12027
Size
100μl
Form
Liquid
Description
Boster Bio Anti-RCL1 Antibody catalog # A12027. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RCL1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12027)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
F7E4
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human RCL1. AA range:324-373
Reactive Species
A12027 is reactive to RCL1 in Human, Mouse, Rat
Calculated molecular weight
40.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12027 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
Validation Images & Assay Conditions
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Western Blot (WB) analysis of HuvEc cells using RCL1 Polyclonal antibody.
Specific Publications For Anti-RCL1 Antibody (A12027)
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Customer Q&As
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3 Customer Q&As for Anti-RCL1 Antibody
Question
Does anti-RCL1 antibody A12027 work on primate WB with umbilical cord blood?
Verified Customer
Verified customer
Asked: 2018-03-08
Answer
Our lab technicians have not validated anti-RCL1 antibody A12027 on primate. You can run a BLAST between primate and the immunogen sequence of anti-RCL1 antibody A12027 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate umbilical cord blood in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-03-08
Question
I have attached the WB image, lot number and protocol we used for seminoma using anti-RCL1 antibody A12027. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-02-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-02-22
Question
We are currently using anti-RCL1 antibody A12027 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
R. Zhao
Verified customer
Asked: 2018-02-14
Answer
The anti-RCL1 antibody (A12027) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-14
