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Product Info Summary
| SKU: | A05686-2 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-REG3A Antibody (C-term)
SKU/Catalog Number
A05686-2
Size
80 µl
Description
Boster Bio Anti-REG3A Antibody (C-term) (Catalog # A05686-2). Tested in Flow Cytometry, WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-REG3A Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05686-2)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This REG3A antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 129-163 amino acids from the C-terminal region of human REG3A.
Reactive Species
A05686-2 is reactive to REG3A in Human
Calculated molecular weight
19.4 kDa
Background of REG3A
Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. Regulates keratinocyte proliferation and differentiation after skin injury via activation of EXTL3-PI3K-AKT signaling pathway.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05686-2 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
FC: 1:25
Validation Images & Assay Conditions
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Western blot analysis of lysate from human pancreas tissue lysate, using REG3A Antibody (C-term). A05686-2 was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
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Flow cytometric analysis of HepG2 cells using REG3A Antibody (C-term)(green, ) compared to an isotype control of rabbit IgG(blue). A05686-2 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Specific Publications For Anti-REG3A Antibody (C-term) (A05686-2)
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