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Product Info Summary
| SKU: | A05495 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Relaxin-3 RLN3 Antibody
SKU/Catalog Number
A05495
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Relaxin-3 RLN3 Antibody (Catalog # A05495). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
RLN3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Relaxin-3 RLN3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05495)
Host
Rabbit
Contents
RLN3 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
RLN3 antibody was raised against a 19 amino acid peptide near the center of human RLN3. The immunogen is located within amino acids 80 - 130 of RLN3.
Cross-reactivity
RLN3 antibody is human specific. RLN3 antibody is predicted to not cross-react with other members of the Relaxin protein family.
Reactive Species
A05495 is reactive to RLN3 in Human
Observed Molecular Weight
68 kDa
Background of RLN3
Relaxin-3 (RLN3) is a member of Relaxin family, a group of endocrine and autocrine/paracrine hormones that belong to the insulin gene superfamily (1,2). RLN3 can bind to and activate three relaxin-family peptide receptors: RXFP1, RXFP3, and RXFP4 (3). In contrast to the widespread expression of the related proteins RLN1 and RLN2, RLN3 is predominantly expressed in brain and to a lesser extent in the thymus, kidney and spleen (1). RLN3 has been suggested to play a role in feeding, metabolism, stress, arousal, learning, and memory (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05495 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
RLN3 antibody can be used for detection of RLN3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of RLN3 in HeLa cell lysate with RLN3 antibody at (A) 1 and (B) 2 μg/ml.
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Immunohistochemistry of RLN3 in human brain tissue with RLN3 antibody at 5 μg/mL.
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Immunofluorescence of RLN3 in human brain tissue with RLN3 antibody at 20 μg/mL.
Specific Publications For Anti-Relaxin-3 RLN3 Antibody (A05495)
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