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Product Info Summary
| SKU: | A07556-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-REQUIEM DPF2 Antibody
SKU/Catalog Number
A07556-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-REQUIEM DPF2 Antibody (Catalog # A07556-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
REQUIEM antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-REQUIEM DPF2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07556-1)
Host
Rabbit
Contents
REQUIEM Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
REQUIEM antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human Requiem. The immunogen is located within the last 50 amino acids of REQUIEM.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Requiem antibody is predicted to not cross-react with other DPF protein family members.
Reactive Species
A07556-1 is reactive to DPF2 in Human, Mouse
Applications
A07556-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
44.155kDa
Background of DPF2
Requiem is a member of the d4 domain family, characterized by a zinc finger-like structural motif. This protein functions as a transcription factor which is necessary for the apoptotic response following deprivation of survival factors. It is expressed in numerous tissues in both cytosolic and nuclear regions and likely serves a regulatory role in rapid hematopoietic cell growth and turnover. Requiem has been found to act as an adaptor protein that links the NF-κB and SWI/SNF chromatin remodeling factor and is considered a candidate gene for multiple endocrine neoplasia type I, an inherited cancer syndrome involving multiple parathyroid, enteropancreatic, and pituitary tumors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
REQUIEM antibody can be used for detection of REQUIEM by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Requiem in mouse kidney tissue lysate with Requiem antibody at 1 μg/mL.
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Immunohistochemistry of REQUIEM in human kidney tissue with REQUIEM antibody at 5 μg/mL.
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Immunofluorescence of REQUIEM in human kidney tissue with REQUIEM antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For DPF2 (Source: Uniprot.org, NCBI)
Gene Name
DPF2
Full Name
Zinc finger protein ubi-d4
Weight
44.155kDa
Superfamily
requiem/DPF family
Alternative Names
Apoptosis response zinc finger protein; D4, zinc and double PHD fingers family 2BAF45D; MGC10180; Protein requiem; REQBRG1-associated factor 45D; requiem, apoptosis response zinc finger; ubi-d4; UBID4requiem, apoptosis response zinc finger gene; zinc finger protein ubi-d4 DPF2 CSS7, REQ, UBID4, ubi-d4 double PHD fingers 2 zinc finger protein ubi-d4|BAF45D|BRG1-associated factor 45D|D4, zinc and double PHD fingers family 2|apoptosis response zinc finger protein|protein requiem|requiem, apoptosis response zinc finger
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DPF2, check out the DPF2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DPF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-REQUIEM DPF2 Antibody (A07556-1)
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Customer Reviews
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