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Product Info Summary
|Application:||IF, IHC, ICC, WB|
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Anti-Retinoic Acid Receptor beta RARB Monoclonal Antibody
Boster Bio Anti-Retinoic Acid Receptor beta RARB Monoclonal Antibody catalog # M01912. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Retinoic Acid Receptor beta RARB Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01912)
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
A synthesized peptide derived from human Retinoic Acid Receptor beta Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
M01912 is reactive to RARB in Human
M01912 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of RARB using anti-RARB antibody (M01912) in MCF7 cell lysate.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RARB antigen affinity purified polyclonal antibody (Catalog # M01912) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Gene/Protein Information For RARB (Source: Uniprot.Org, NCBI)
Retinoic acid receptor beta
nuclear hormone receptor family
beta polypeptide; HAP; HBV-activated protein; NR1B2; RAR beta; RARB; retinoic acid receptor, beta; RRB2 RARB HAP, MCOPS12, NR1B2, RARbeta1, RRB2 retinoic acid receptor beta retinoic acid receptor beta|HBV-activated protein|RAR-beta|RAR-epsilon|hepatitis B virus activated protein|nuclear receptor subfamily 1 group B member 2|retinoic acid receptor, beta polypeptide*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on RARB, check out the RARB Infographic
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In this infographic you will see the following information for RARB: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for M01912
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