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Product Info Summary
| SKU: | A08516-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-RFX4 Antibody Picoband®
SKU/Catalog Number
A08516-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-RFX4 Antibody Picoband® catalog # A08516-2. Tested in WB, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-RFX4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08516-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived human RFX4 recombinant protein (Position: Q124-H667). Human RFX4 shares 96.7% amino acid (aa) sequence identity with mouse RFX4.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A08516-2 is reactive to RFX4 in Human
Observed Molecular Weight
90 kDa
Calculated molecular weight
83.4 kDa
Background of RFX4
Transcription factor RFX4 is a protein that in humans is encoded by the RFX4 gene. This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X1, X2, X3, and X5. It has been shown to interact with itself as well as with regulatory factors X2 and X3, but it does not interact with regulatory factor X1. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08516-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human U-87MG whole cell, human U251 whole cell
Validation Images & Assay Conditions
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Western blot analysis of RFX4 using anti-RFX4 antibody (A08516-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates,
Lane 2: human U251 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RFX4 antigen affinity purified polyclonal antibody (Catalog # A08516-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RFX4 at approximately 90 kDa. The expected band size for RFX4 is at 83 kDa.
Specific Publications For Anti-RFX4 Antibody Picoband® (A08516-2)
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