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Product Info Summary
| SKU: | A18306 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-RGPD5 Antibody
SKU/Catalog Number
A18306
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-RGPD5 Antibody (Catalog # A18306). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
RGPD5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-RGPD5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A18306)
Host
Rabbit
Contents
RGPD5 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
RGPD5 antibody was raised against a 13 amino acid synthetic peptide from near the amino terminus of human RGPD5. The immunogen is located within amino acids 50 - 100 of RGPD5.
Reactive Species
A18306 is reactive to RGPD5 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
198.9 kDa
Background of RGPD5
The RANBP2-like and GRIP domain containing 5 protein (RGPD5) has high similarity to RANBP2, a large RAN-binding protein localized at the cytoplasmic side of the nuclear pore complex. The gene coding for RGPD5 is thought to have arisen from a gene duplication event of RANBP2 as these highly homologous genes are located close to each other at chromosome 2q11-q12. RGPD5 was identified as an HIV dependency factor (HDF), suggesting that RGPD5 may be an important drug target in HIV treatment. At least two isoforms of RGPD5 are known to exist, of which the shorter isoform is expressed primarily in testis, while the longer of the two is expressed at low levels in a number of somatic tissues.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A18306 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
RGPD5 antibody can be used for detection of RGPD5 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of RGPD5 in human thymus tissue lysate with RGPD5 antibody at 1 μg/mL.
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Immunohistochemistry of RGPD5 in human testis tissue cells with RGPD5 antibody at 10 μg/mL.
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Immunofluorescence of RGPD5 in human testis tissue with RGPD5 antibody at 20 μg/mL.
Specific Publications For Anti-RGPD5 Antibody (A18306)
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