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Product Info Summary
| SKU: | PA2050 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-RGS3 Antibody
SKU/Catalog Number
PA2050
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-RGS3 Antibody catalog # PA2050. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RGS3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2050)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse RGS3, identical to the related rat sequence, and different from the related human sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2050 is reactive to RGS3 in Human, Mouse, Rat
Applications
PA2050 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
93 kDa
Calculated molecular weight
132.336kDa
Background of RGS3
RGS3 (Regulator of G Protein Signaling 3), is a protein that in humans is encoded by the RGS3 gene. Chatterjee et al. (1997) studied RGS3, the largest member of the RGS family then identified. Mouse Pdz-Rgs3 can mediate signaling from the ephrin-B cytoplasmic tail. By radiation hybrid mapping, Chatterjee et al. (1997) assigned the RGS3 gene to chromosome 9q31-q33. Lu et al. (2001) showed that Sdf1, a chemokine with a G protein-coupled receptor, and Bdnf are chemoattractants for cerebellar granule cells, and that Sdf1 chemoattraction is selectively inhibited by soluble ephrin-B receptor. This inhibition could be blocked by a truncated Pdz-Rgs3 protein lacking the RGS domain.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Validation Images & Assay Conditions
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Anti-RGS3 antibody, PA2050, Western blotting
Lane 1: Rat Testis Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Spleen Tissue Lysate
Lane 4: HELA Cell Lysate
Lane 5: U87 Cell Lysate
Click image to see more details
Anti-RGS3 antibody, PA2050, IHC(P)
IHC(P): Rat Intestine Tissue
Protein Target Info & Infographic
Gene/Protein Information For RGS3 (Source: Uniprot.org, NCBI)
Gene Name
RGS3
Full Name
Regulator of G-protein signaling 3
Weight
132.336kDa
Alternative Names
C2PA; FLJ20370; FLJ31516; FLJ90496; PDZ-RGS3; regulator of G-protein signaling 3; regulator of G-protein signalling 3; RGP3 RGS3 C2PA, RGP3 regulator of G protein signaling 3 regulator of G-protein signaling 3|regulator of G-protein signalling 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RGS3, check out the RGS3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RGS3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RGS3 Antibody (PA2050)
Hello CJ!
No publications found for PA2050
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
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- Boster's Pathways Library
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Customer Reviews
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*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
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Find answers in Q&As, reviews.
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4 Customer Q&As for Anti-RGS3 Antibody
Question
Does anti-RGS3 antibody PA2050 work on feline WB with brain kidney?
Verified Customer
Verified customer
Asked: 2019-01-18
Answer
Our lab technicians have not validated anti-RGS3 antibody PA2050 on feline. You can run a BLAST between feline and the immunogen sequence of anti-RGS3 antibody PA2050 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline brain kidney in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-01-18
Question
We are currently using anti-RGS3 antibody PA2050 for rat tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2017-05-31
Answer
The anti-RGS3 antibody (PA2050) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-05-31
Question
I see that the anti-RGS3 antibody PA2050 works with IHC, what is the protocol used to produce the result images on the product page?
N. Rodriguez
Verified customer
Asked: 2016-12-09
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-12-09
Question
Will PA2050 anti-RGS3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
J. Yang
Verified customer
Asked: 2014-09-30
Answer
As indicated on the product datasheet, PA2050 anti-RGS3 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-09-30
