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Product Info Summary
| SKU: | A05056 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-RHAMM HMMR Antibody
SKU/Catalog Number
A05056
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-RHAMM HMMR Antibody (Catalog # A05056). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
RHAMM antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-RHAMM HMMR Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05056)
Host
Rabbit
Contents
RHAMM Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
RHAMM antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human RHAMM. The immunogen is located within amino acids 80 - 130 of RHAMM.
Reactive Species
A05056 is reactive to HMMR in Human, Mouse, Rat
Observed Molecular Weight
35 kDa
Calculated molecular weight
84.1 kDa
Background of HMMR
The hyaluronan-mediated motility receptor, also known as RHAMM, was initially identified as a soluble protein that could be released by sub-confluent migrating cells, promoting cell motility and invasion via interactions with hyaluronan (HA) and the cell surface. While RHAMM is normally poorly expressed in most normal tissues and is not required for embryonic development or normal cell homeostasis functions, its expression is increased during wound repair in response to hypoxia and fibrogenic factors. However, its overexpression is transforming in multiple types of cancers and is required for maintaining RAS transformation. RHAMM associates with BRCA1 and BARD1, attenuating the mitotic-spindle-promoting activity of RHAMM, which may contribute to tumor progression by promoting genomic instability.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05056 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
RHAMM antibody can be used for detection of RHAMM by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Validation Images & Assay Conditions
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RHAMM KO Validation in HeLa Cells
Loading: 10 μg of lysate
Antibodies: XRHAMM A05056, 1 μg/mL and beta-actin 3779-1301, 1μg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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Western blot analysis of RHAMM in rat stomach tissue lysate with RHAMM antibody at 0.5 μg/mL.
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Immunohistochemistry of RHAMM in human stomach tissue with RHAMM antibody at 2.5 μg/mL.
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Immunofluorescence of RHAMM in human stomach tissue with RHAM antibody at 20 μg/mL.
Specific Publications For Anti-RHAMM HMMR Antibody (A05056)
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