Product Info Summary
SKU: | PA1960 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, IHC-F, ICC, WB |
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Product info
Product Name
Anti-RIAM/APBB1IP Antibody
View all RIAM/APBB1IP Antibodies
SKU/Catalog Number
PA1960
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-RIAM/APBB1IP Antibody catalog # PA1960. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RIAM/APBB1IP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1960)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human APBB1IP, different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1960 is reactive to APBB1IP in Human, Mouse, Rat
Applications
PA1960 is guaranteed for Flow Cytometry, IF, IHC, IHC-F, ICC, WB Boster Guarantee
Observed Molecular Weight
110-120 kDa
Calculated molecular weight
73.183kDa
Background of RIAM/APBB1IP
APBB1IP (APBB1-Interacting Protein), also called RIAM or RARP1, is a protein that in humans is encoded by the APBB1IP gene. By genomic sequence analysis, Lafuente et al. (2004) mapped the RIAM gene to chromosome 10p12.1. Using promoter-reporter gene assays, Inagaki et al. (2003) found that RARP1 suppressed transcription from AP1 and SRE sites, but not CRE sites, in all cell lines examined. The proline-rich regions of RARP1 suppressed AP1 transactivation. Lafuente et al. (2004) found that RIAM interacted with profilin and VASP, molecules that regulate actin dynamics, as well as with RAP1-GTP.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat
Immunocytochemistry , 0.5-1μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of APBB1IP using anti-APBB1IP antibody (PA1960 ).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: Jurkat whole cell lysates,
Lane 3: human Raji whole cell lysates,
Lane 4: U937 whole cell lysates,
Lane 5: rat thymus tissue lysates,
Lane 6: mouse thymus tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APBB1IP antigen affinity purified polyclonal antibody (Catalog # PA1960 ) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APBB1IP at approximately 110-120KD. The expected band size for APBB1IP is at 73KD.
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Anti-RIAM antibody, PA1960, ICC
ICC: JURKAT Cell
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Anti-RIAM antibody, PA1960, IHC(P)
IHC(P): Human Tonsil Tissue
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Figure 4. IHC analysis of APBB1IP using anti-APBB1IP antibody (PA1960). APBB1IP was detected in paraffin-embedded section of rat lymphaden. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-APBB1IP Antibody (PA1960) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 5. IHC analysis of APBB1IP using anti-APBB1IP antibody (PA1960). APBB1IP was detected in frozen section of rat spleen. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with μg/ml rabbit anti-APBB1IP Antibody (PA1960) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 6. IF analysis of APBB1IP using anti-APBB1IP antibody (PA1960).
APBB1IP was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-APBB1IP Antibody (PA1960) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 7. Flow Cytometry analysis of SiHa cells using anti-APBB1IP antibody (PA1960).
Overlay histogram showing SiHa cells stained with PA1960 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-APBB1IP Antibody (PA1960, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For APBB1IP (Source: Uniprot.org, NCBI)
Gene Name
APBB1IP
Full Name
Amyloid beta A4 precursor protein-binding family B member 1-interacting protein
Weight
73.183kDa
Superfamily
MRL family
Alternative Names
amyloid beta (A4) precursor protein-binding, family B, member 1 interactingprotein; amyloid beta A4 precursor protein-binding family B member 1-interacting protein; APBB1-interacting protein 1; APBB1IP; INAG1; PREL1; PREL-1; proline rich EVH1 ligand 1; Proline-rich EVH1 ligand 1; Proline-rich protein 73; Rap1-GTP-interacting adapter molecule; Rap1-interacting adaptor molecule; RARP1; Retinoic acid-responsive proline-rich protein 1; RIAM; RIAMRARP-1 APBB1IP INAG1, PREL1, RARP1, RIAM amyloid beta precursor protein binding family B member 1 interacting protein amyloid beta A4 precursor protein-binding family B member 1-interacting protein|APBB1-interacting protein 1|PREL-1|RARP-1|Rap1-GTP-interacting adaptor molecule|Rap1-interacting adaptor molecule|proline rich EVH1 ligand 1|proline-rich protein 73|rap1-GTP-interacting adapter molecule|retinoic acid-responsive proline-rich protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on APBB1IP, check out the APBB1IP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for APBB1IP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RIAM/APBB1IP Antibody (PA1960)
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No publications found for PA1960
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-RIAM/APBB1IP Antibody
Question
I see that the anti-RIAM/APBB1IP antibody PA1960 works with ICC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-06
Question
Is this PA1960 anti-RIAM/APBB1IP antibody reactive to the isotypes of APBB1IP?
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
The immunogen of PA1960 anti-RIAM/APBB1IP antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human APBB1IP(647-666aa EQDFMSDLMKALQKKRGNVS), different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-16
Question
We are currently using anti-RIAM/APBB1IP antibody PA1960 for rat tissue, and we are content with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
G. Wu
Verified customer
Asked: 2015-10-12
Answer
The anti-RIAM/APBB1IP antibody (PA1960) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-10-12