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Product Info Summary
| SKU: | A07057-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Ribosomal Protein L18 RPL18 Antibody
SKU/Catalog Number
A07057-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Ribosomal Protein L18 RPL18 Antibody catalog # A07057-1. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ribosomal Protein L18 RPL18 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07057-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human RPL18. AA range:111-160
Reactive Species
A07057-1 is reactive to RPL18 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
21.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07057-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot (WB) analysis of specific cells using Ribosomal Protein L18 Polyclonal antibody.
Specific Publications For Anti-Ribosomal Protein L18 RPL18 Antibody (A07057-1)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Ribosomal Protein L18 RPL18 Antibody
Question
We are currently using anti-Ribosomal Protein L18 antibody A07057-1 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
L. Edwards
Verified customer
Asked: 2019-04-19
Answer
The anti-Ribosomal Protein L18 antibody (A07057-1) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-19
Question
I was wanting to use your anti-Ribosomal Protein L18 antibody for WB for rat cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat cervix carcinoma identification?
Z. Carter
Verified customer
Asked: 2019-01-28
Answer
You can see on the product datasheet, A07057-1 anti-Ribosomal Protein L18 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-01-28
Question
See below the WB image, lot number and protocol we used for cervix carcinoma using anti-Ribosomal Protein L18 antibody A07057-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-12-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-03
Question
Does A07057-1 anti-Ribosomal Protein L18 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-05-18
Answer
You can see on the product datasheet, A07057-1 anti-Ribosomal Protein L18 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-05-18
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-Ribosomal Protein L18 antibody A07057-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-06-21
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-06-21
Question
Is a blocking peptide available for product anti-Ribosomal Protein L18 antibody (A07057-1)?
Z. Parker
Verified customer
Asked: 2017-03-21
Answer
We do provide the blocking peptide for product anti-Ribosomal Protein L18 antibody (A07057-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-03-21
Question
Is this A07057-1 anti-Ribosomal Protein L18 antibody reactive to the isotypes of RPL18?
D. Kulkarni
Verified customer
Asked: 2013-11-13
Answer
The immunogen of A07057-1 anti-Ribosomal Protein L18 antibody is Synthesized peptide derived from the Internal region of human Ribosomal Protein L18. at AA rangle: 80-160. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-11-13
