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Product Info Summary
| SKU: | A13318 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, ICC, WB |
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Product info
Product Name
Anti-RING finger protein 121 RNF121 Antibody
SKU/Catalog Number
A13318
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-RING finger protein 121 RNF121 Antibody (Catalog # A13318). Tested in ELISA, WB, ICC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
RNF121 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-RING finger protein 121 RNF121 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13318)
Host
Rabbit
Contents
RNF121 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
RNF121 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human RNF121. The immunogen is located within amino acids 260 - 310 of RNF121.
Cross-reactivity
RNF121 antibody is human, mouse and rat reactive. Multiple isoforms of RNF121 are known to exist.
Reactive Species
A13318 is reactive to RNF121 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
37.9 kDa
Background of RNF121
RNF121 is an evolutionarily conserved E3 ligase ring finger protein that is expressed in the endoplasmic reticulum (1). It acts downstream of the unfolded protein response regulator protein kinase-like endoplasmic reticulum kinase (PERK). Overexpression of RNF121 during the early stages of gonad development led to aberrations in germline development and gonad migration, suggesting that it plays a role in endoplasmic reticulum-associated degradation machinery (1).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13318 is guaranteed for ELISA, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
RNF121 antibody can be used for detection of RNF121 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunocytochemistry at 10 μg/ml.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of RNF121 in MCF7 cell lysate with RNF121 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Immunocytochemistry of RNF121 in MCF7 cells with RNF121 antibody at 10 μg/ml.
Specific Publications For Anti-RING finger protein 121 RNF121 Antibody (A13318)
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