Anti-RLBP1 Antibody Picoband™

Product Name

Anti-RLBP1 Antibody Picoband™

View all CRALBP Antibodies

SKU/Catalog Number

A05421-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-RLBP1 Antibody Picoband™ catalog # A05421-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-RLBP1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05421-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human RLBP1 recombinant protein (Position: E3-D288).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A05421-1 is reactive to RLBP1 in Human, Mouse, Rat

Applications

A05421-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

36 kDa

Calculated molecular weight

36.474kDa

Background of CRALBP

Retinaldehyde-binding protein 1 (RLBP1) also known as cellular retinaldehyde-binding protein (CRALBP) is a 36-kD water-soluble protein that in humans is encoded by the RLBP1 gene. The protein encoded by this gene is a 36-kD water-soluble protein which carries 11-cis-retinaldehyde or 11-cis-retinal as physiologic ligands. It may be a functional component of the visual cycle. Mutations of this gene have been associated with severe rod-cone dystrophy, Bothnia dystrophy (nonsyndromic autosomal recessive retinitis pigmentosa) and retinitis punctata albescens.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Mouse, Rat
Flow Cytometry, 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of RLBP1 using anti-RLBP1 antibody (A05421-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat eye tissue lysates,
Lane 2: mouse eye tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RLBP1 antigen affinity purified polyclonal antibody (Catalog # A05421-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RLBP1 at approximately 36 kDa. The expected band size for RLBP1 is at 36 kDa.

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Figure 2. Flow Cytometry analysis of HepG2 cells using anti-RLBP1 antibody (A05421-1).
Overlay histogram showing HepG2 cells stained with A05421-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RLBP1 Antibody (A05421-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For RLBP1 (Source: Uniprot.org, NCBI)

Alternative Names

Cellular retinaldehyde-binding protein; cellular retinaldehyde-binding protein-1; CRALBPretinaldehyde-binding protein 1; MGC3663; retinaldehyde binding protein 1 RLBP1 CRALBP retinaldehyde binding protein 1 retinaldehyde-binding protein 1|cellular retinaldehyde-binding protein|cellular retinaldehyde-binding protein-1

For more info on RLBP1, check out the RLBP1 Infographic

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In this infographic, you will see the following information for RLBP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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