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Product Info Summary
| SKU: | A18242 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-RING finger protein 113B RNF113B Antibody
SKU/Catalog Number
A18242
Size
100μl
Form
Liquid
Description
Boster Bio Anti-RING finger protein 113B RNF113B Antibody catalog # A18242. Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RING finger protein 113B RNF113B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A18242)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human RNF113B.
Reactive Species
A18242 is reactive to RNF113B in Human
Calculated molecular weight
36.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A18242 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200 (room temperature, 45min).
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Western Blot analysis of 293 cells using RNF113B Polyclonal Antibody
Specific Publications For Anti-RING finger protein 113B RNF113B Antibody (A18242)
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Customer Q&As
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5 Customer Q&As for Anti-RING finger protein 113B RNF113B Antibody
Question
My lab would like to test anti-RNF113B antibody A18242 on human testis for research purposes, then I may be interested in using anti-RNF113B antibody A18242 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
The products we sell, including anti-RNF113B antibody A18242, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-29
Question
Can you help my question with product A18242, anti-RNF113B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-09-18
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A18242 anti-RNF113B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-09-18
Question
We are currently using anti-RNF113B antibody A18242 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2017-11-01
Answer
The anti-RNF113B antibody (A18242) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-01
Question
Is a blocking peptide available for product anti-RNF113B antibody (A18242)?
T. Dhar
Verified customer
Asked: 2017-08-09
Answer
We do provide the blocking peptide for product anti-RNF113B antibody (A18242). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-08-09
Question
I see that the anti-RNF113B antibody A18242 works with WB, what is the protocol used to produce the result images on the product page?
H. Krishna
Verified customer
Asked: 2016-02-02
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2016-02-02
