Anti-RNF144b/Ibrdc2 Antibody

IBRDC2 antibody

Boster Bio Anti-RNF144b/Ibrdc2 Antibody catalog # A10490. Tested in WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A10490
Size: 100μl
Reactive Species: Human, Mouse
Host: Rabbit
Application: WB

Product Name

Anti-RNF144b/Ibrdc2 Antibody

View all IBRDC2 Antibodies

SKU/Catalog Number

A10490

Size

100μl

Form

Liquid

Description

Boster Bio Anti-RNF144b/Ibrdc2 Antibody catalog # A10490. Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-RNF144b/Ibrdc2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10490)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human p53RFP.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A10490 is reactive to Rnf144b in Human, Mouse

Applications

A10490 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

33.495kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For Rnf144b (Source: Uniprot.org, NCBI)

Gene Name

Rnf144b

Full Name

E3 ubiquitin-protein ligase RNF144B

Weight

33.495kDa

Superfamily

RBR family

Alternative Names

bA528A10.3; E3 ubiquitin-protein ligase RNF144B; EC 6.3.2; EC 6.3.2.-; IBR domain containing 2; IBR domain-containing protein 2; IBRDC2; KIAA0161; p53-inducible RING finger protein; p53RFP; PIR2; ring finger protein 144BMGC71786

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Rnf144b, check out the Rnf144b Infographic

Rnf144b infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Rnf144b: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-RNF144b/Ibrdc2 Antibody

Question

Is this A10490 anti-RNF144b/Ibrdc2 antibody reactive to the isotypes of RNF144B?

Verified Customer

Verified customer

Asked: 2020-04-28

Answer

The immunogen of A10490 anti-RNF144b/Ibrdc2 antibody is Synthesized peptide derived from the Internal region of human p53RFP. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-04-28

Question

My question regards to test anti-RNF144b/Ibrdc2 antibody A10490 on mouse quadriceps femoris for research purposes, then I may be interested in using anti-RNF144b/Ibrdc2 antibody A10490 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-10-31

Answer

The products we sell, including anti-RNF144b/Ibrdc2 antibody A10490, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-10-31

Question

We are currently using anti-RNF144b/Ibrdc2 antibody A10490 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2019-07-01

Answer

The anti-RNF144b/Ibrdc2 antibody (A10490) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-01

Question

Would anti-RNF144b/Ibrdc2 antibody A10490 work on bovine WB with prostate testis?

Verified Customer

Verified customer

Asked: 2019-06-25

Answer

Our lab technicians have not tested anti-RNF144b/Ibrdc2 antibody A10490 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-RNF144b/Ibrdc2 antibody A10490 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine prostate testis in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-06-25

Question

I was wanting to use your anti-RNF144b/Ibrdc2 antibody for WB for mouse quadriceps femoris on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse quadriceps femoris identification?

Verified Customer

Verified customer

Asked: 2019-06-14

Answer

It shows on the product datasheet, A10490 anti-RNF144b/Ibrdc2 antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse quadriceps femoris in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-06-14

Question

Is a blocking peptide available for product anti-RNF144b/Ibrdc2 antibody (A10490)?

Verified Customer

Verified customer

Asked: 2019-03-06

Answer

We do provide the blocking peptide for product anti-RNF144b/Ibrdc2 antibody (A10490). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-03-06

Question

Does A10490 anti-RNF144b/Ibrdc2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

T. Jackson

Verified customer

Asked: 2016-01-07

Answer

It shows on the product datasheet, A10490 anti-RNF144b/Ibrdc2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2016-01-07

Order DetailsPrice
A10490

100uL

$399

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A10490
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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