Anti-RNF186 Antibody Picoband™

RNF186 antibody

Boster Bio Anti-RNF186 Antibody Picoband™ catalog # A15259-1. Tested in ELISA, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A15259-1
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA, WB

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Product Name

Anti-RNF186 Antibody Picoband™

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SKU/Catalog Number

A15259-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-RNF186 Antibody Picoband™ catalog # A15259-1. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-RNF186 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15259-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human RNF186 recombinant protein (Position: R86-L159).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A15259-1 is reactive to RNF186 in Human

Applications

A15259-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

28 kDa

Calculated molecular weight

24.145kDa

Background of RNF186

RNF186 is an E3 ubiquitin ligase that regulates endoplasmic reticulum (ER) stress and apoptosis through its interaction with BNIP1. This gene is mapped to chromosome 1p36.13 based on an alignment of theRNF186 sequence with the genomic sequence (GRCh38). Cotransfection and immunoprecipitation analyses showed that RNF186, through it transmembrane domains, interacted with BNIP1. Overexpression of RNF186 promoted BNIP1 ubiquitination and transfer of BNIP1 to mitochondria.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For RNF186 (Source: Uniprot.org, NCBI)

Gene Name

RNF186

Full Name

E3 ubiquitin-protein ligase RNF186

Weight

24.145kDa

Alternative Names

FLJ20225; ring finger protein 186; RP11-91K11.1 RNF186 ring finger protein 186 E3 ubiquitin-protein ligase RNF186

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on RNF186, check out the RNF186 Infographic

RNF186 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for RNF186: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-RNF186 Antibody Picoband™

Question

Will anti-RNF186 antibody A15259-1 work on canine WB with colon mucosa?

Verified Customer

Verified customer

Asked: 2020-04-17

Answer

Our lab technicians have not validated anti-RNF186 antibody A15259-1 on canine. You can run a BLAST between canine and the immunogen sequence of anti-RNF186 antibody A15259-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine colon mucosa in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-04-17

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney pancreas using anti-RNF186 antibody A15259-1. Let me know if you need anything else.

B. Baker

Verified customer

Asked: 2020-02-07

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-07

Question

Is this A15259-1 anti-RNF186 antibody reactive to the isotypes of RNF186?

Verified Customer

Verified customer

Asked: 2019-12-16

Answer

The immunogen of A15259-1 anti-RNF186 antibody is E. coli-derived human RNF186 recombinant protein (Position: R86-L159). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-12-16

Question

We are currently using anti-RNF186 antibody A15259-1 for human tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?

E. Baker

Verified customer

Asked: 2013-05-06

Answer

The anti-RNF186 antibody (A15259-1) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-05-06

Order DetailsPrice
A15259-1

100μg

$370
A15259-1-10ug

10μg sample (liquid)

$99
A15259-1-Biotin

100 μg Biotin conjugated

$570
A15259-1-Cy3

100 μg Cy3 conjugated

$570
A15259-1-Dylight488

100 μg Dylight488 conjugated

$570
A15259-1-Dylight550

100 μg Dylight550 conjugated

$570
A15259-1-Dylight594

100 μg Dylight594 conjugated

$570
A15259-1-FITC

100 μg FITC conjugated

$570
A15259-1-HRP

100 μg HRP conjugated

$570
A15259-1-APC

100 μg APC conjugated

$670
A15259-1-PE

100 μg PE conjugated

$670
A15259-1-carrier-free

Carrier Free

$370

More conjugates/formats

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A15259-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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