Anti-ROBO-1 Antibody

ROBO1 antibody

Boster Bio Anti-ROBO-1 Antibody catalog # A01530-1. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A01530-1
Size: 100ug
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC, WB

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Product Name

Anti-ROBO-1 Antibody

View all ROBO1 Antibodies

SKU/Catalog Number

A01530-1

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-ROBO-1 Antibody catalog # A01530-1. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Cite This Product

Anti-ROBO-1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01530-1)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality

Polyclonal

Immunogen

This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1632-1644 of Human ROBO-1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A01530-1 is reactive to ROBO1 in Human, Mouse, Rat

Applications

A01530-1 is guaranteed for IF, IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

IF Microscopy: User Optimized
Western Blot: 1:500 - 1:3,000
Immunohistochemistry: 2 ug/ml to 10 ug/ml

Validation Images & Assay Conditions

Gene/Protein Information For ROBO1 (Source: Uniprot.org, NCBI)

Uniprot ID

Q9Y6N7

Gene ID

6091

Gene Name

ROBO1

Full Name

Roundabout homolog 1

Weight

180.93kDa

Superfamily

immunoglobulin superfamily

Alternative Names

DUTT1; DUTT1Drosophila) homolog 1; H-Robo-1; MGC131599; MGC133277; ROBO1; roundabout, axon guidance receptor, homolog 1 (Drosophila) ROBO1 DUTT1, SAX3 roundabout guidance receptor 1 roundabout homolog 1|deleted in U twenty twenty|roundabout, axon guidance receptor, homolog 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ROBO1, check out the ROBO1 Infographic

ROBO1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ROBO1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

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6 Customer Q&As for Anti-ROBO-1 Antibody

Question

Will A01530-1 anti-ROBO-1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-02-24

Answer

It shows on the product datasheet, A01530-1 anti-ROBO-1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-02-24

Question

Is this A01530-1 anti-ROBO-1 antibody reactive to the isotypes of ROBO1?

Verified Customer

Verified customer

Asked: 2020-01-28

Answer

The immunogen of A01530-1 anti-ROBO-1 antibody is This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1632-1644 of Human ROBO-1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-01-28

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for forebrain using anti-ROBO-1 antibody A01530-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-12-11

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-11

Question

Is a blocking peptide available for product anti-ROBO-1 antibody (A01530-1)?

Verified Customer

Verified customer

Asked: 2018-06-21

Answer

We do provide the blocking peptide for product anti-ROBO-1 antibody (A01530-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-06-21

Question

See below the WB image, lot number and protocol we used for forebrain using anti-ROBO-1 antibody A01530-1. Please let me know if you require anything else.

K. Bhatt

Verified customer

Asked: 2014-05-27

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-05-27

Question

Our lab want to know about to test anti-ROBO-1 antibody A01530-1 on human forebrain for research purposes, then I may be interested in using anti-ROBO-1 antibody A01530-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

M. Carter

Verified customer

Asked: 2014-03-03

Answer

The products we sell, including anti-ROBO-1 antibody A01530-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2014-03-03

Size

Total: $487

SKU:A01530-1

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A01530-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$487.00

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