Anti-ROBO4 Antibody Picoband™

Product Name

Anti-ROBO4 Antibody Picoband™

View all ROBO4 Antibodies

SKU/Catalog Number

A04479-2

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-ROBO4 Antibody Picoband™ catalog # A04479-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-ROBO4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04479-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human ROBO4 recombinant protein (Position: D108-A933).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A04479-2 is reactive to ROBO4 in Human, Mouse, Rat

Applications

A04479-2 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

107 kDa

Calculated molecular weight

107.457kDa

Background of ROBO4

Roundabout homolog 4 is a protein that in humans is encoded by the ROBO4 gene. Predicted to enable cell-cell adhesion mediator activity. Involved in angiogenesis and establishment of endothelial barrier. Located in extracellular exosome. Implicated in aortic valve disease 3.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of ROBO4 using anti-ROBO4 antibody (A04479-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: rat PC-12 whole cell lysates,
Lane 4: mouse LLC whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROBO4 antigen affinity purified polyclonal antibody (Catalog # A04479-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROBO4 at approximately 107 kDa. The expected band size for ROBO4 is at 90-170 kDa.

Gene/Protein Information For ROBO4 (Source: Uniprot.org, NCBI)

Alternative Names

ECSM4; FLJ20798; Magic roundabout; MGC133352; MGC133353; MRB; ROBO4; roundabout homolog 4; roundabout homolog 4, magic roundabout (Drosophila) ROBO4 AOVD3, ECSM4, MRB roundabout guidance receptor 4 roundabout homolog 4|roundabout homolog 4, magic roundabout|roundabout, axon guidance receptor, homolog 4

For more info on ROBO4, check out the ROBO4 Infographic

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In this infographic, you will see the following information for ROBO4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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