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Product Info Summary
| SKU: | M01023-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-ROCK2 Antibody (C-term)
SKU/Catalog Number
M01023-1
Size
80 µl
Description
Boster Bio Anti-ROCK2 Antibody (C-term) (Catalog # M01023-1). Tested in WB application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-ROCK2 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01023-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This ROCK2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1346-1380 amino acids from the C-terminal region of human ROCK2.
Reactive Species
M01023-1 is reactive to ROCK2 in Human, Mouse, Rat
Calculated molecular weight
160.9 kDa
Background of ROCK2
Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01023-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
Validation Images & Assay Conditions
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All lanes : Anti-ROCK2 Antibody (C-term) at 1:2000 dilution
Lane 1: NCI-H1299 whole cell lysates
Lane 2: C2C12 whole cell lysates
Lane 3: C6 whole cell lysates
Lane 4: MCF-7 whole cell lysates
Lane 5: Hela whole cell lysates
Lane 6: HT-1080 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 161 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-ROCK2 Antibody (C-term) (M01023-1)
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