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Product Info Summary
| SKU: | A06498 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Roquin-1 RC3H1 Antibody
SKU/Catalog Number
A06498
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Roquin-1 RC3H1 Antibody (Catalog # A06498). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
RC3H1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Roquin-1 RC3H1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06498)
Host
Rabbit
Contents
RC3H1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Rabbit polyclonal RC3H1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human RC3H1. The immunogen is located within amino acids 1040 - 1090 of RC3H1.
Cross-reactivity
At least three isoforms of RC3H1 are known to exist; this antibody will detect all three isoforms.
Reactive Species
A06498 is reactive to RC3H1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
125.7 kDa
Background of RC3H1
The ring finger protein RC3H1, also known as Roquin, is a highly conserved member of the RING type ubiquitin ligase protein family whose M199R mutation leads to the excessive production of follicular helper T cells and germinal centers in the sanroque strain of mice, a strain with excessive IL-21 production and high titers of autoantibodies. The complete loss of RC3H1 induces early death and immune deregulation but not autoimmunity in RC3H1-null mice, suggesting that the mutant RC3H1 is more disruptive to the immune system than its complete loss.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06498 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
RC3H1 antibody can be used for detection of RC3H1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of RC3H1 in HeLa cell lysate with RC3H1 antibody at 1 μg/mL
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Immunohistochemistry of RC3H1 in human small intestine tissue with RC3H1 antibody at 5 μg/ml.
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Immunofluorescence of RC3H1 in human small intestine tissue with RC3H1 antibody at 20 μg/ml.
Specific Publications For Anti-Roquin-1 RC3H1 Antibody (A06498)
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