Product Info Summary
SKU: | A01923-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-RP2 Antibody Picoband™
SKU/Catalog Number
A01923-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-RP2 Antibody Picoband™ catalog # A01923-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RP2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01923-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human RP2 recombinant protein (Position: D244-M348).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01923-1 is reactive to RP2 in Human, Mouse, Rat
Applications
A01923-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
40 kDa
Calculated molecular weight
39.641kDa
Background of RP2
Protein XRP2 is a protein that in humans is encoded by the RP2 gene. It is mapped to Xp11.3. The RP2 locus has been implicated as one cause of X-linked retinitis pigmentosa. The predicted gene product shows homology with human cofactor C, a protein involved in the ultimate step of beta-tubulin folding. Progressive retinal degeneration may therefore be due to the accumulation of incorrectly folded photoreceptor or neuron-specific tubulin isoforms followed by progressive cell death. The RP2 protein is also involved in regulating the function and extension of outer segment of cone photoreceptors in mice.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of RP2 using anti-RP2 antibody (A01923-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human SGC-7901 whole cell lysate,
Lane 3: rat lung tissue lysates,
Lane 4: mouse lung tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RP2 antigen affinity purified polyclonal antibody (Catalog # A01923-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RP2 at approximately 40KD. The expected band size for RP2 is at 40KD.
Click image to see more details
Figure 10. IF analysis of RP2 using anti-RP2 antibody (A01923-1).
RP2 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-RP2 Antibody (A01923-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 2. IHC analysis of RP2 using anti-RP2 antibody (A01923-1).
RP2 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RP2 Antibody (A01923-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of RP2 using anti-RP2 antibody (A01923-1).
RP2 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RP2 Antibody (A01923-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of RP2 using anti-RP2 antibody (A01923-1).
RP2 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RP2 Antibody (A01923-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of RP2 using anti-RP2 antibody (A01923-1).
RP2 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RP2 Antibody (A01923-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 6. IHC analysis of RP2 using anti-RP2 antibody (A01923-1).
RP2 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RP2 Antibody (A01923-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 7. IHC analysis of RP2 using anti-RP2 antibody (A01923-1).
RP2 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RP2 Antibody (A01923-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 8. Flow Cytometry analysis of A431 cells using anti-RP2 antibody (A01923-1).
Overlay histogram showing A431 cells stained with A01923-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RP2 Antibody (A01923-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 9. Flow Cytometry analysis of THP-1 cells using anti-RP2 antibody (A01923-1).
Overlay histogram showing THP-1 cells stained with A01923-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RP2 Antibody (A01923-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For RP2 (Source: Uniprot.org, NCBI)
Gene Name
RP2
Full Name
Protein XRP2
Weight
39.641kDa
Superfamily
TBCC family
Alternative Names
DELXp11.3; KIAA0215; NME10; protein XRP2; retinitis pigmentosa 2 (X-linked recessive); TBCCD2; XRP2 RP2 DELXp11.3, NM23-H10, NME10, TBCCD2, XRP2 RP2 activator of ARL3 GTPase protein XRP2|RP2, ARL3 GTPase activating protein|retinitis pigmentosa 2 (X-linked recessive)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RP2, check out the RP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RP2 Antibody Picoband™ (A01923-1)
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No publications found for A01923-1
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-RP2 Antibody Picoband™
Question
Will anti-RP2 antibody A01923-1 work for Flow Cytometry with bone marrow?
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
According to the expression profile of bone marrow, RP2 is highly expressed in bone marrow. So, it is likely that anti-RP2 antibody A01923-1 will work for Flow Cytometry with bone marrow.
Boster Scientific Support
Answered: 2020-03-13
Question
Will anti-RP2 antibody A01923-1 work on primate Flow Cytometry with bone marrow?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
Our lab technicians have not validated anti-RP2 antibody A01923-1 on primate. You can run a BLAST between primate and the immunogen sequence of anti-RP2 antibody A01923-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate bone marrow in Flow Cytometry, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-20
Question
Is a blocking peptide available for product anti-RP2 antibody (A01923-1)?
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
We do provide the blocking peptide for product anti-RP2 antibody (A01923-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-23
Question
Is this A01923-1 anti-RP2 antibody reactive to the isotypes of RP2?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
The immunogen of A01923-1 anti-RP2 antibody is E. coli-derived human RP2 recombinant protein (Position: D244-M348). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-17
Question
Our lab want to know about to test anti-RP2 antibody A01923-1 on rat bone marrow for research purposes, then I may be interested in using anti-RP2 antibody A01923-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
B. Jones
Verified customer
Asked: 2018-10-15
Answer
The products we sell, including anti-RP2 antibody A01923-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-10-15