Anti-RP2 Antibody Picoband™ (monoclonal, 3D7)

Product Name

Anti-RP2 Antibody Picoband™ (monoclonal, 3D7)

View all RP2 Antibodies

SKU/Catalog Number

M01923

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-RP2 Antibody Picoband™ (monoclonal, 3D7) catalog # M01923. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-RP2 Antibody Picoband™ (monoclonal, 3D7) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01923)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Monoclonal

Clone Number

3D7

Isotype

Mouse IgG2b

Immunogen

E. coli-derived human RP2recombinant protein (Position: D244-M348).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M01923 is reactive to RP2 in Human, Mouse, Rat

Applications

M01923 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

40 kDa

Calculated molecular weight

39.641kDa

Background of RP2

Protein XRP2 is a protein that in humans is encoded by the RP2 gene. It is mapped to Xp11.3. The RP2 locus has been implicated as one cause of X-linked retinitis pigmentosa. The predicted gene product shows homology with human cofactor C, a protein involved in the ultimate step of beta-tubulin folding. Progressive retinal degeneration may therefore be due to the accumulation of incorrectly folded photoreceptor or neuron-specific tubulin isoforms followed by progressive cell death.The RP2 protein is also involved in regulating the function and extension of outer segment of cone photoreceptors in mice.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/ml, Human
Flow Cytometry, 1-3μg/1x10⁶ cells, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of RP2 using anti ZO-1 antibody (M01923).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HepG2 tissue lysates,
Lane 2: human Jurkat, whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: human A549 whole cell lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-RP2 antigen affinity purified polyclonal antibody (Catalog # M01923) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for RP2 at approximately 40KD. The expected band size for RP2 is at 40KD.

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Figure 2. IF analysis of RP2 using anti-RP2 antibody (M01923).
RP2 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-RP2 Antibody (M01923) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 3. Flow Cytometry analysis of 293T cells using anti-RP2 antibody M01923).
Overlay histogram showing 293T cells stained with M01923 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-RP2 Antibody (M01923, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For RP2 (Source: Uniprot.org, NCBI)

Alternative Names

DELXp11.3; KIAA0215; NME10; protein XRP2; retinitis pigmentosa 2 (X-linked recessive); TBCCD2; XRP2 RP2 DELXp11.3, NM23-H10, NME10, TBCCD2, XRP2 RP2 activator of ARL3 GTPase protein XRP2|RP2, ARL3 GTPase activating protein|retinitis pigmentosa 2 (X-linked recessive)

For more info on RP2, check out the RP2 Infographic

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In this infographic, you will see the following information for RP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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