Anti-RPA135 POLR1B Antibody

Product Name

Anti-RPA135 POLR1B Antibody

View all Polr1b Antibodies

SKU/Catalog Number

A11908-1

Size

100ul

Form

Liquid

Description

Boster Bio Anti-RPA135 POLR1B Antibody catalog # A11908-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-RPA135 POLR1B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11908-1)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human RPA135.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A11908-1 is reactive to Polr1b in Human, Mouse, Rat

Applications

A11908-1 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

128.213kDa

Background of Polr1b

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft By similarity.

Ota T., Nat. Genet. 36:40-45(2004).
Hillier L.W., Nature 434:724-731(2005).
Miller G., EMBO J. 20:1373-1382(2001).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

Gene/Protein Information For Polr1b (Source: Uniprot.org, NCBI)

Alternative Names

DNA-directed RNA polymerase I 135 kDa polypeptide; DNA-directed RNA polymerase I 135kDa polypeptide; DNA-directed RNA polymerase I subunit RPA2; EC 2.7.7.6; FLJ10816; FLJ21921; MGC131780; polymerase (RNA) I polypeptide B, 128kDa; RNA polymerase I subunit 2; RPA116; RPA135; RPA2; Rpo1-2 Polr1b|128kD, 128kDa, D630020H17Rik, R, RPA, RPA116, RPA135, RPA2, Rpo, Rpo1-2|polymerase (RNA) I polypeptide B|DNA-directed RNA polymerase I subunit RPA2|DNA-directed RNA polymerase I 135 kDa polypeptide|DNA-directed RNA polymerase I 135kDa polypeptide|RNA polymerase 1-2, 128 kDa subunit|RNA polymerase I subunit 2

For more info on Polr1b, check out the Polr1b Infographic

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In this infographic, you will see the following information for Polr1b: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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