Product Info Summary
SKU: | A04696 |
---|---|
Size: | 100μl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-RPA14 RPA3 Antibody
SKU/Catalog Number
A04696
Size
100μl
Form
Liquid
Description
Boster Bio Anti-RPA14 RPA3 Antibody catalog # A04696. Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RPA14 RPA3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04696)
Host
Rabbit
Contents
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide from human protein at AA range: 30-80.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04696 is reactive to RPA3 in Human, Mouse
Applications
A04696 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
13.569kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-2000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of SW480, MCF7 lysate, antibody was diluted at 2000. Secondary antibody was diluted at 1:20000.
Protein Target Info & Infographic
Gene/Protein Information For RPA3 (Source: Uniprot.org, NCBI)
Gene Name
RPA3
Full Name
Replication protein A 14 kDa subunit
Weight
13.569kDa
Alternative Names
REPA3Replication factor A protein 3; replication protein A 14 kDa subunit; replication protein A3 (14kD); replication protein A3, 14kDa; RF-A protein 3; RP-A p14; RPA14 RPA3 REPA3, RP-A p14 replication protein A3 replication protein A 14 kDa subunit|RF-A protein 3|replication factor A protein 3|replication protein A3, 14kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RPA3, check out the RPA3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RPA3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RPA14 RPA3 Antibody (A04696)
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No publications found for A04696
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Customer Reviews
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Customer Q&As
Have a question?
Find answers in Q&As, reviews.
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13 Customer Q&As for Anti-RPA14 RPA3 Antibody
Question
My question regarding product A04696, anti-RPA14 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-19
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04696 anti-RPA14 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-19
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney lung using anti-RPA14 antibody A04696. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-14
Question
I am interested in to test anti-RPA14 antibody A04696 on human kidney lung for research purposes, then I may be interested in using anti-RPA14 antibody A04696 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
The products we sell, including anti-RPA14 antibody A04696, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-06
Question
Will A04696 anti-RPA14 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Roberts
Verified customer
Asked: 2018-09-04
Answer
You can see on the product datasheet, A04696 anti-RPA14 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-09-04
Question
I was wanting to use your anti-RPA14 antibody for WB for human kidney lung on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human kidney lung identification?
Verified Customer
Verified customer
Asked: 2018-06-05
Answer
It shows on the product datasheet, A04696 anti-RPA14 antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human kidney lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-06-05
Question
Is this A04696 anti-RPA14 antibody reactive to the isotypes of RPA3?
Verified Customer
Verified customer
Asked: 2018-05-18
Answer
The immunogen of A04696 anti-RPA14 antibody is Synthetic peptide from human protein at AA range: 30-80. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-05-18
Question
Does anti-RPA14 antibody A04696 work on horse WB with liver?
Verified Customer
Verified customer
Asked: 2018-04-30
Answer
Our lab technicians have not tested anti-RPA14 antibody A04696 on horse. You can run a BLAST between horse and the immunogen sequence of anti-RPA14 antibody A04696 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-04-30
Question
We are currently using anti-RPA14 antibody A04696 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on pig tissues as well?
M. Yang
Verified customer
Asked: 2017-12-20
Answer
The anti-RPA14 antibody (A04696) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-12-20
Question
Here is the WB image, lot number and protocol we used for kidney lung using anti-RPA14 antibody A04696. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-08-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-08-25
Question
Would anti-RPA14 antibody A04696 work for WB with kidney lung?
H. Parker
Verified customer
Asked: 2017-01-09
Answer
According to the expression profile of kidney lung, RPA3 is highly expressed in kidney lung. So, it is likely that anti-RPA14 antibody A04696 will work for WB with kidney lung.
Boster Scientific Support
Answered: 2017-01-09
Question
Do you have a BSA free version of anti-RPA14 antibody A04696 available?
J. Li
Verified customer
Asked: 2015-09-14
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-RPA14 antibody A04696 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-09-14
Question
I see that the anti-RPA14 antibody A04696 works with WB, what is the protocol used to produce the result images on the product page?
N. Miller
Verified customer
Asked: 2015-07-16
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-07-16
Question
Is a blocking peptide available for product anti-RPA14 antibody (A04696)?
S. Collins
Verified customer
Asked: 2013-06-14
Answer
We do provide the blocking peptide for product anti-RPA14 antibody (A04696). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-06-14