Click image to see more details
Product Info Summary
| SKU: | A30655 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-RPAB1 Antibody
SKU/Catalog Number
A30655
Size
100ul
Form
Liquid
Description
Boster Bio Anti-RPAB1 Antibody (Catalog# A30655). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RPAB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30655)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human RPAB1. Immunogen sequence location: 21-70
Reactive Species
A30655 is reactive to POLR2E in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
24.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30655 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:40000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Click image to see more details
Western blot analysis of lysates from MCF-7 cells, using RPAB1 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-RPAB1 Antibody (A30655)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-RPAB1 Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-RPAB1 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Anti-RPAB1 Antibody
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for heart left ventricle using anti-RPAB1 antibody A30655. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-03
Question
Does anti-RPAB1 antibody A30655 work for WB with heart left ventricle?
Verified Customer
Verified customer
Asked: 2019-07-15
Answer
According to the expression profile of heart left ventricle, POLR2E is highly expressed in heart left ventricle. So, it is likely that anti-RPAB1 antibody A30655 will work for WB with heart left ventricle.
Boster Scientific Support
Answered: 2019-07-15
Question
I see that the anti-RPAB1 antibody A30655 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-07-03
Question
We are currently using anti-RPAB1 antibody A30655 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-12-14
Answer
The anti-RPAB1 antibody (A30655) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-14
Question
Is this A30655 anti-RPAB1 antibody reactive to the isotypes of POLR2E?
G. Carter
Verified customer
Asked: 2017-12-18
Answer
The immunogen of A30655 anti-RPAB1 antibody is A peptide derived from human RPAB1. Immunogen sequence location: 21-70. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-12-18
