Anti-RPAB1 Antibody

POLR2E antibody

Boster Bio Anti-RPAB1 Antibody (Catalog# A30655). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A30655
Size: 100ul
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IHC-P, WB, IF (paraffin)

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Product Name

Anti-RPAB1 Antibody

View all POLR2E Antibodies

SKU/Catalog Number

A30655

Size

100ul

Form

Liquid

Description

Boster Bio Anti-RPAB1 Antibody (Catalog# A30655). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-RPAB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30655)

Host

Rabbit

Contents

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A peptide derived from human RPAB1. Immunogen sequence location: 21-70

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A30655 is reactive to POLR2E in Human, Mouse, Rat

Applications

A30655 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

24.551kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:40000
Not yet tested in other applications.

Validation Images & Assay Conditions

Gene/Protein Information For POLR2E (Source: Uniprot.org, NCBI)

Gene Name

POLR2E

Full Name

DNA-directed RNA polymerases I, II, and III subunit RPABC1

Weight

24.551kDa

Superfamily

archaeal RpoH/eukaryotic RPB5 RNA polymerase subunit family

Alternative Names

DNA directed RNA polymerase II 23 kda polypeptide; DNA-directed RNA polymerase II 23 kDa polypeptide; DNA-directed RNA polymerase II subunit E; DNA-directed RNA polymerases I, II, and III subunit RPABC1; hRPB25; hsRPB5; polymerase (RNA) II (DNA directed) polypeptide E (25kD); polymerase (RNA) II (DNA directed) polypeptide E, 25kDa; RPABC1; RPB5 homolog; RPB5; XAP4RNA polymerases I, II, and III subunit ABC1 POLR2E RPABC1, RPB5, XAP4, hRPB25, hsRPB5 RNA polymerase II, I and III subunit E DNA-directed RNA polymerases I, II, and III subunit RPABC1|DNA directed RNA polymerase II 23 kda polypeptide|DNA-directed RNA polymerase II 23 kDa polypeptide|DNA-directed RNA polymerase II subunit E|DNA-directed RNA polymerase subunit RPABC1|RNA polymerase II subunit E|RNA polymerases I, II, and III subunit ABC1|RPB5 homolog|polymerase (RNA) II (DNA directed) polypeptide E, 25kDa|polymerase (RNA) II subunit E

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on POLR2E, check out the POLR2E Infographic

POLR2E infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for POLR2E: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-RPAB1 Antibody

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for heart left ventricle using anti-RPAB1 antibody A30655. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-01-03

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-01-03

Question

Does anti-RPAB1 antibody A30655 work for WB with heart left ventricle?

Verified Customer

Verified customer

Asked: 2019-07-15

Answer

According to the expression profile of heart left ventricle, POLR2E is highly expressed in heart left ventricle. So, it is likely that anti-RPAB1 antibody A30655 will work for WB with heart left ventricle.

Boster Scientific Support

Answered: 2019-07-15

Question

I see that the anti-RPAB1 antibody A30655 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-07-03

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-07-03

Question

We are currently using anti-RPAB1 antibody A30655 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2018-12-14

Answer

The anti-RPAB1 antibody (A30655) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-12-14

Question

Is this A30655 anti-RPAB1 antibody reactive to the isotypes of POLR2E?

G. Carter

Verified customer

Asked: 2017-12-18

Answer

The immunogen of A30655 anti-RPAB1 antibody is A peptide derived from human RPAB1. Immunogen sequence location: 21-70. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-12-18

Order DetailsPrice
A30655

100 μL

$335

Free Secondary Antibody

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A30655
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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