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Product Info Summary
| SKU: | A03391-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-RPAP3 Antibody Picoband®
SKU/Catalog Number
A03391-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-RPAP3 Antibody Picoband® catalog # A03391-1. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-RPAP3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03391-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived human RPAP3 recombinant protein (Position: R84-E604). Human RPAP3 shares 78.5% and 77.9% amino acid (aa) sequence identity with mouse and rat RPAP3, respectively.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A03391-1 is reactive to RPAP3 in Human, Mouse, Rat
Observed Molecular Weight
76 kDa
Calculated molecular weight
75.7 kDa
Background of RPAP3
This gene encodes an RNA polymerase II-associated protein. The encoded protein may function in transcriptional regulation and may also regulate apoptosis. Alternatively spliced transcript variants have been described.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03391-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat,ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human A549 whole cell, human THP-1 whole cell, human K562 whole cell, human Caco-2 whole cell, rat PC-12 whole cell, rat NRK whole cell, mouse RAW2647 whole cell
Validation Images & Assay Conditions
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Western blot analysis of RPAP3 using anti-RPAP3 antibody (A03391-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: rat NRK whole cell lysates,
Lane 7: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPAP3 antigen affinity purified polyclonal antibody (Catalog # A03391-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPAP3 at approximately 76 kDa. The expected band size for RPAP3 is at 76 kDa.
Specific Publications For Anti-RPAP3 Antibody Picoband® (A03391-1)
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