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Product Info Summary
| SKU: | A05272-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-RPE Antibody Picoband®
SKU/Catalog Number
A05272-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-RPE Antibody Picoband® catalog # A11762. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-RPE Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05272-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived human RPE recombinant protein (Position: D68-E110). Human RPE shares 97.7% amino acid (aa) sequence identity mouse RPE.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A05272-1 is reactive to RPE in Human, Mouse, Rat
Observed Molecular Weight
25 kDa
Calculated molecular weight
24.9 kDa
Background of RPE
Ribulose-phosphate 3-epimerase is an enzyme that in humans is encoded by the RPE gene. RPE catalyzes the reversible epimerization of D-ribulose 5- phosphate to D-xylulose 5-phosphate. This gene is belongs to the ribulose-phosphate 3-epimerase family. 2 isoforms of the human protein are produced by alternative splicing.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05272-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human 293T whole cell, human Hela whole cell, human K562 whole cell, human HepG2 whole cell, rat thymus tissue, rat RH35 whole cell, mouse thymus tissue, mouse NIH/3T3 whole cell
Validation Images & Assay Conditions
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Western blot analysis of RPE using anti-RPE antibody (A05272-1).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat thymus tissue lysates,
Lane 6: rat RH35 whole cell lysates,
Lane 7: mouse thymus tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPE antigen affinity purified polyclonal antibody (Catalog # A05272-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RPE at approximately 25 kDa. The expected band size for RPE is at 25 kDa.
Specific Publications For Anti-RPE Antibody Picoband® (A05272-1)
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