Anti-RPL15/Ribosomal Protein L15 Antibody

RPL15 antibody

Boster Bio Anti-RPL15/Ribosomal Protein L15 Antibody (Catalog # A07136). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A07136
Size: 100 µl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-RPL15/Ribosomal Protein L15 Antibody

View all RPL15 Antibodies

SKU/Catalog Number

A07136

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-RPL15/Ribosomal Protein L15 Antibody (Catalog # A07136). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-RPL15/Ribosomal Protein L15 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07136)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human RPL15.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A07136 is reactive to RPL15 in Human, Mouse, Rat

Applications

A07136 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

24.146kDa

Background of RPL15

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L15E family of ribosomal proteins. It is located in the cytoplasm. This gene shares sequence similarity with the yeast ribosomal protein YL10 gene. Although this gene has been referred to as RPL10, its official symbol is RPL15. This gene has been shown to be overexpressed in some esophageal tumors compared to normal matched tissues. Alternate splicing results in multiple transcript variants. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Herzog H.; Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases.
Wang Q., Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases.
Yoshihama M., Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For RPL15 (Source: Uniprot.org, NCBI)

Gene Name

RPL15

Full Name

60S ribosomal protein L15

Weight

24.146kDa

Superfamily

eukaryotic ribosomal protein eL15 family

Alternative Names

60S ribosomal protein L15; EC45MGC88603; FLJ26304; ribosomal protein L15; RPL10; RPLY10; RPYL10 RPL15 DBA12, EC45, L15, RPL10, RPLY10, RPYL10 ribosomal protein L15 60S ribosomal protein L15|large ribosomal subunit protein eL15

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on RPL15, check out the RPL15 Infographic

RPL15 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for RPL15: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-RPL15/Ribosomal Protein L15 Antibody

Question

Does A07136 anti-RPL15/Ribosomal Protein L15 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-08-06

Answer

As indicated on the product datasheet, A07136 anti-RPL15/Ribosomal Protein L15 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-08-06

Question

Do you have a BSA free version of anti-RPL15/Ribosomal Protein L15 antibody A07136 available?

Verified Customer

Verified customer

Asked: 2018-12-19

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-RPL15/Ribosomal Protein L15 antibody A07136 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-12-19

Question

I see that the anti-RPL15/Ribosomal Protein L15 antibody A07136 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-09-26

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-09-26

Question

We are currently using anti-RPL15/Ribosomal Protein L15 antibody A07136 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2018-01-17

Answer

The anti-RPL15/Ribosomal Protein L15 antibody (A07136) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-01-17

Order DetailsPrice
A07136-100uL

100uL

$360

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