Anti-RPL34/Ribosomal Protein L34 Antibody

RPL34 antibody

Boster Bio Anti-RPL34/Ribosomal Protein L34 Antibody (Catalog # A08190). Tested in WB, IF applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A08190
Size: 100 µl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, WB

Product Name

Anti-RPL34/Ribosomal Protein L34 Antibody

View all RPL34 Antibodies

SKU/Catalog Number

A08190

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-RPL34/Ribosomal Protein L34 Antibody (Catalog # A08190). Tested in WB, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-RPL34/Ribosomal Protein L34 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08190)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Immunogen

Synthesized peptide derived from internal of human RPL34.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A08190 is reactive to RPL34 in Human, Mouse, Rat

Applications

A08190 is guaranteed for IF, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

13.293kDa

Background of RPL34

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L34E family of ribosomal proteins. It is located in the cytoplasm. This gene originally was thought to be located at 17q21, but it has been mapped to 4q. Transcript variants derived from alternative splicing, alternative transcription initiation sites, and/or alternative polyadenylation exist; these variants encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Rommens J.M., Genomics 28:530-542(1995).
Yoshihama M., Genome Res. 12:379-390(2002).
Kenmochi N., Genome Res. 8:509-523(1998).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Immunofluorescence: 1:100~1:500

Validation Images & Assay Conditions

Gene/Protein Information For RPL34 (Source: Uniprot.org, NCBI)

Gene Name

RPL34

Full Name

60S ribosomal protein L34

Weight

13.293kDa

Superfamily

eukaryotic ribosomal protein eL34 family

Alternative Names

leukemia-associated protein; M; MGC111005; ribosomal protein L34,60S ribosomal protein L34 RPL34 L34 ribosomal protein L34 60S ribosomal protein L34|large ribosomal subunit protein eL34|leukemia-associated protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on RPL34, check out the RPL34 Infographic

RPL34 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for RPL34: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-RPL34/Ribosomal Protein L34 Antibody

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for bone eye using anti-RPL34/Ribosomal Protein L34 antibody A08190. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-10-22

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-22

Question

Would A08190 anti-RPL34/Ribosomal Protein L34 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-03-22

Answer

It shows on the product datasheet, A08190 anti-RPL34/Ribosomal Protein L34 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-03-22

Question

We are currently using anti-RPL34/Ribosomal Protein L34 antibody A08190 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2018-01-22

Answer

The anti-RPL34/Ribosomal Protein L34 antibody (A08190) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-01-22

Question

Would anti-RPL34/Ribosomal Protein L34 antibody A08190 work on primate WB with liver?

B. Williams

Verified customer

Asked: 2016-08-15

Answer

Our lab technicians have not tested anti-RPL34/Ribosomal Protein L34 antibody A08190 on primate. You can run a BLAST between primate and the immunogen sequence of anti-RPL34/Ribosomal Protein L34 antibody A08190 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate liver in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2016-08-15

Question

Is there a BSA free version of anti-RPL34/Ribosomal Protein L34 antibody A08190 available?

M. Krishna

Verified customer

Asked: 2013-05-10

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-RPL34/Ribosomal Protein L34 antibody A08190 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2013-05-10

Order DetailsPrice
A08190-100uL

100uL

$360

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A08190
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$360.00

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