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Product Info Summary
| SKU: | A06221-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-RPS13/Ribosomal Protein S13 Antibody
SKU/Catalog Number
A06221-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-RPS13/Ribosomal Protein S13 Antibody (Catalog# A06221-1). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RPS13/Ribosomal Protein S13 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06221-1)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human RPS13. Immunogen sequence location: 71-120
Reactive Species
A06221-1 is reactive to RPS13 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
17.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06221-1 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:40000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Western blot analysis of lysates from PC12 cells, primary antibody was diluted at 1:1000, 4°over night
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using RPS13 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-RPS13/Ribosomal Protein S13 Antibody (A06221-1)
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Customer Q&As
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6 Customer Q&As for Anti-RPS13/Ribosomal Protein S13 Antibody
Question
Will anti-RPS13/Ribosomal Protein S13 antibody A06221-1 work on goat IHC with bone?
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
Our lab technicians have not tested anti-RPS13/Ribosomal Protein S13 antibody A06221-1 on goat. You can run a BLAST between goat and the immunogen sequence of anti-RPS13/Ribosomal Protein S13 antibody A06221-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat bone in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-29
Question
See below the WB image, lot number and protocol we used for uterus using anti-RPS13/Ribosomal Protein S13 antibody A06221-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-05
Question
Is a blocking peptide available for product anti-RPS13/Ribosomal Protein S13 antibody (A06221-1)?
Verified Customer
Verified customer
Asked: 2018-12-25
Answer
We do provide the blocking peptide for product anti-RPS13/Ribosomal Protein S13 antibody (A06221-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-12-25
Question
We are currently using anti-RPS13/Ribosomal Protein S13 antibody A06221-1 for rat tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
Z. Johnson
Verified customer
Asked: 2017-09-28
Answer
The anti-RPS13/Ribosomal Protein S13 antibody (A06221-1) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-09-28
Question
Is this A06221-1 anti-RPS13/Ribosomal Protein S13 antibody reactive to the isotypes of RPS13?
K. Krishna
Verified customer
Asked: 2017-09-14
Answer
The immunogen of A06221-1 anti-RPS13/Ribosomal Protein S13 antibody is A peptide derived from human RPS13. Immunogen sequence location: 71-120. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-09-14
Question
I see that the anti-RPS13/Ribosomal Protein S13 antibody A06221-1 works with IHC, what is the protocol used to produce the result images on the product page?
B. Evans
Verified customer
Asked: 2014-06-10
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2014-06-10
