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Product Info Summary
| SKU: | M05831 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-RPS17 Antibody (Center)
SKU/Catalog Number
M05831
Size
50 µl
Description
Boster Bio Anti-RPS17 Antibody (Center) (Catalog # M05831). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-RPS17 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05831)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This RPS17 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 55-87 amino acids from the Central region of human RPS17.
Reactive Species
M05831 is reactive to RPS17 in Human
Calculated molecular weight
15.6 kDa
Background of RPS17
Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs and xenobiotic compounds. Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites. Sulfates hydroxysteroids like DHEA. Isoform 1 preferentially sulfonates cholesterol, and isoform 2 avidly sulfonates pregnenolone but not cholesterol. Plays a role in epidermal cholesterol metabolism and in the regulation of epidermal proliferation and differentiation (PubMed:28575648).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05831 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
Validation Images & Assay Conditions
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All lanes : Anti-RPS17 Antibody (Center) at 1:2000 dilution
Lane 1: Jurkat whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: MCF-7 whole cell lysate
Lane 4: HL-60 whole cell lysate
Lane 5: THP-1 whole cell lysate
Lane 6: NCI-H292 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 16 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-RPS17 Antibody (Center) (M05831)
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