Anti-RPS4X Antibody

RPS4X antibody

Boster Bio Anti-RPS4X Antibody (Catalog # A09096). Tested in WB, IF applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A09096
Size: 100 µl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, WB

Product Name

Anti-RPS4X Antibody

View all RPS4X Antibodies

SKU/Catalog Number

A09096

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-RPS4X Antibody (Catalog # A09096). Tested in WB, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-RPS4X Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09096)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human RPS4X.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A09096 is reactive to RPS4X in Human, Mouse, Rat

Applications

A09096 is guaranteed for IF, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

29.598kDa

Background of RPS4X

Cytoplasmic ribosomes, organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes ribosomal protein S4, a component of the 40S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene and ribosomal protein S4, Y-linked (RPS4Y). The 2 isoforms encoded by these genes are not identical, but are functionally equivalent. Ribosomal protein S4 belongs to the S4E family of ribosomal proteins. This gene is not subject to X-inactivation. It has been suggested that haploinsufficiency of the ribosomal protein S4 genes plays a role in Turner syndrome; however, this hypothesis is controversial. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Fisher E.M.C., Cell 63:1205-1218(1990).
Watanabe M., J. Cell Sci. 100:35-43(1991).
The MGC Project Team; Genome Res. 14:2121-2127(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Immunofluorescence: 1:100~1:500

Validation Images & Assay Conditions

Gene/Protein Information For RPS4X (Source: Uniprot.org, NCBI)

Gene Name

RPS4X

Full Name

40S ribosomal protein S4, X isoform

Weight

29.598kDa

Superfamily

eukaryotic ribosomal protein eS4 family

Alternative Names

CCG2Single copy abundant mRNA protein; DXS306; FLJ40595; ribosomal protein S4, X-linked; RPS4cell cycle gene 2; SCAR40S ribosomal protein S4, X isoform; SCR10ribosomal protein S4X isoform; single-copy abundant mRNA RPS4X CCG2, DXS306, RPS4, S4, SCAR, SCR10 ribosomal protein S4 X-linked 40S ribosomal protein S4, X isoform|cell cycle gene 2|single copy abundant mRNA protein|single-copy abundant mRNA|small ribosomal subunit protein eS4

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on RPS4X, check out the RPS4X Infographic

RPS4X infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for RPS4X: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-RPS4X Antibody

Question

Does anti-RPS4X antibody A09096 work on goat WB with germinal epithelium of ovary?

Verified Customer

Verified customer

Asked: 2020-04-08

Answer

Our lab technicians have not validated anti-RPS4X antibody A09096 on goat. You can run a BLAST between goat and the immunogen sequence of anti-RPS4X antibody A09096 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat germinal epithelium of ovary in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-04-08

Question

We are currently using anti-RPS4X antibody A09096 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2020-02-19

Answer

The anti-RPS4X antibody (A09096) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-02-19

Question

I was wanting to use your anti-RPS4X antibody for IF for mouse germinal epithelium of ovary on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse germinal epithelium of ovary identification?

Verified Customer

Verified customer

Asked: 2020-02-13

Answer

You can see on the product datasheet, A09096 anti-RPS4X antibody has been tested for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse germinal epithelium of ovary in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-02-13

Question

Is this A09096 anti-RPS4X antibody reactive to the isotypes of RPS4X?

Verified Customer

Verified customer

Asked: 2018-02-20

Answer

The immunogen of A09096 anti-RPS4X antibody is Synthesized peptide derived from internal of human RPS4X. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-02-20

Question

Would anti-RPS4X antibody A09096 work for IF with germinal epithelium of ovary?

Z. Parker

Verified customer

Asked: 2013-01-11

Answer

According to the expression profile of germinal epithelium of ovary, RPS4X is highly expressed in germinal epithelium of ovary. So, it is likely that anti-RPS4X antibody A09096 will work for IF with germinal epithelium of ovary.

Boster Scientific Support

Answered: 2013-01-11

Order DetailsPrice
A09096-100uL

100uL

$360

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A09096
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$360.00

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