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Product Info Summary
| SKU: | M04736 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-RPS7 Antibody (C-Term)
SKU/Catalog Number
M04736
Size
50 µl
Description
Boster Bio Anti-RPS7 Antibody (C-Term) (Catalog # M04736). Tested in WB, Flow Cytometry, IF application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-RPS7 Antibody (C-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04736)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This RPS7 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 158-191 amino acids from human RPS7.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04736 is reactive to RPS7 in Human, Mouse, Rat
Applications
M04736 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
22.127kDa
Background of RPS7
Required for rRNA maturation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:2000
FC: 1:25
Validation Images & Assay Conditions
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling RPS7 with M04736 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image mainly showing cytoplasm staining on HeLa cell line. The nuclear counter stain is DAPI (blue).
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All lanes : Anti-RPS7 Antibody (C-Term) at 1:2000 dilution
Lane 1: Hela whole cell lysate
Lane 2: A549 whole cell lysate
Lane 3: HepG2 whole cell lysate
Lane 4: Jurkat whole cell lysate
Lane 5: NIH/3T3 whole cell lysate
Lane 6: PC-12 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 22 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing Hela cells stained with M04736 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M04736, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Protein Target Info & Infographic
Gene/Protein Information For RPS7 (Source: Uniprot.org, NCBI)
Gene Name
RPS7
Full Name
40S ribosomal protein S7
Weight
22.127kDa
Superfamily
eukaryotic ribosomal protein eS7 family
Alternative Names
DBA8,40S ribosomal protein S7; ribosomal protein S7 RPS7 DBA8, S7, eS7 ribosomal protein S7 40S ribosomal protein S7|small ribosomal subunit protein eS7
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RPS7, check out the RPS7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RPS7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RPS7 Antibody (C-Term) (M04736)
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No publications found for M04736
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