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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
|Application:||ELISA, IF, IHC-P, ICC, WB|
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Anti-RSK3/RPS6KA2 Antibody Picoband™
Boster Bio Anti-RSK3/RPS6KA2 Antibody Picoband™ catalog # A06142-1. Tested in ELISA, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RSK3/RPS6KA2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06142-1)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E. coli-derived human RSK3 recombinant protein (Position: H658-L733).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A06142-1 is reactive to RPS6KA2 in Human, Mouse, Rat
A06142-1 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Direct ELISA, 0.1-0.5μg/ml
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of RSK3 using anti-RSK3 antibody (A06142-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human Hela whole cell lysate,
Lane 3: human PC-3 whole cell lysate,
Lane 4: human A431 whole cell lysate,
Lane 5: human K562 whole cell lysate,
Lane 6: human PANC-1 whole cell lysate,
Lane 7: rat testis tissue lysates,
Lane 8: mouse testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RSK3 antigen affinity purified polyclonal antibody (Catalog # A06142-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RSK3 at approximately 90-100KD. The expected band size for RSK3 is at 83KD.
Gene/Protein Information For RPS6KA2 (Source: Uniprot.Org, NCBI)
Ribosomal protein S6 kinase alpha-2
protein kinase superfamily
EC 2.7.11; EC 220.127.116.11; HU-2; MAP kinase-activated protein kinase 1c; MAPK-activated protein kinase 1c; MAPKAP kinase 1c; MAPKAPK1C; MAPKAPK-1c; p90-RSK 2; p90RSK2; p90-RSK3; ribosomal protein S6 kinase alpha 2; ribosomal protein S6 kinase alpha-2; ribosomal protein S6 kinase, 90kD, polypeptide 2; ribosomal protein S6 kinase, 90kDa, polypeptide 2; Ribosomal S6 kinase 3,90 kDa ribosomal protein S6 kinase 2; RPS6KA2; RSK; RSK3; RSK-3; RSK3pp90RSK3; S6K-alpha; S6K-alpha2; S6K-alpha-2 RPS6KA2 HU-2, MAPKAPK1C, RSK, RSK3, S6K-alpha, S6K-alpha2, p90-RSK3, p90RSK2, pp90RSK3 ribosomal protein S6 kinase A2 ribosomal protein S6 kinase alpha-2|MAP kinase-activated protein kinase 1c|MAPK-activated protein kinase 1c|mitogen-activated protein kinase-activated protein kinase 1C|ribosomal S6 kinase 3|ribosomal protein S6 kinase, 90kDa, polypeptide 2*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on RPS6KA2, check out the RPS6KA2 Infographic
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In this infographic you will see the following information for RPS6KA2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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