Product Info Summary
SKU: | A11829 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-RSPH3 Antibody Picoband™
SKU/Catalog Number
A11829
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-RSPH3 Antibody Picoband™ catalog # A11829. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-RSPH3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11829)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human RSPH3 recombinant protein (Position: Q31-H473).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A11829 is reactive to RSPH3 in Human
Applications
A11829 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
70 kDa
Calculated molecular weight
63.687kDa
Background of RSPH3
Radial spoke head protein 3 homolog (RSPH3), also known as radial spoke head-like protein 2 (RSHL2), is a protein that in humans is encoded by the RSPH3 gene. The protein encoded by this gene acts as a protein kinase A anchoring protein. Mutations in this gene cause primary ciliary dyskinesia; a disorder characterized by defects of the axoneme in motile cilia and sperm flagella. The homolog of this gene was first identified in the blue-green algae Chlamydomonas as encoding a radial spoke protein that formed a structural component of motile cilia and flagella. Alternate splicing results in multiple transcript variants encoding distinct isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of RSPH3 using anti-RSPH3 antibody (A11829).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Siha whole cell lysates,
Lane 2: human U87 whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human Hacat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RSPH3 antigen affinity purified polyclonal antibody (Catalog # A11829) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RSPH3 at approximately 70 kDa. The expected band size for RSPH3 is at 64 kDa.
Click image to see more details
Figure 2. Flow Cytometry analysis of K562 cells using anti-RSPH3 antibody (A11829).
Overlay histogram showing K562 cells stained with A11829 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RSPH3 Antibody (A11829, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For RSPH3 (Source: Uniprot.org, NCBI)
Gene Name
RSPH3
Full Name
Radial spoke head protein 3 homolog
Weight
63.687kDa
Superfamily
flagellar radial spoke RSP3 family
Alternative Names
radial spoke 3 homolog (Chlamydomonas); radial spoke head 3 homolog; radial spoke head protein 3 homolog; Radial spoke head-like protein 2; radial spoke protein 3; radial spokehead-like 2; RSHL2; RSP3dJ111C20.1 RSPH3 CILD32, RSHL2, RSP3, dJ111C20.1 radial spoke head 3 radial spoke head protein 3 homolog|A-kinase anchor protein RSPH3|radial spoke 3 homolog|radial spoke head 3 homolog|radial spoke head-like protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RSPH3, check out the RSPH3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RSPH3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RSPH3 Antibody Picoband™ (A11829)
Hello CJ!
No publications found for A11829
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-RSPH3 Antibody Picoband™?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-RSPH3 Antibody Picoband™
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question