Anti-RUFY1 Antibody Picoband™

Product Name

Anti-RUFY1 Antibody Picoband™

View all RUFY1 Antibodies

SKU/Catalog Number

A10201-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-RUFY1 Antibody Picoband™ catalog # A10201-1. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-RUFY1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10201-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human RUFY1 recombinant protein (Position: L20-Q650).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A10201-1 is reactive to RUFY1 in Human

Applications

A10201-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

80 kDa

Calculated molecular weight

79.818kDa

Background of RUFY1

RUN and FYVE domain-containing protein 1 is a protein that in humans is encoded by the RUFY1 gene. This gene encodes a protein that contains a RUN domain and a FYVE-type zinc finger domain. The encoded protein binds to phosphatidylinositol-3-phosphate (PI3P) and plays a role in early endosomal trafficking, tethering and fusion through interactions with small GTPases including Rab4, Rab5 and Rab14. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of RUFY1 using anti-RUFY1 antibody (A10201-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human SiHa whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RUFY1 antigen affinity purified polyclonal antibody (Catalog # A10201-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RUFY1 at approximately 80 kDa. The expected band size for RUFY1 is at 80 kDa.

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Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-RUFY1 antibody (A10201-1).
Overlay histogram showing MCF-7 cells stained with A10201-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RUFY1 Antibody (A10201-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For RUFY1 (Source: Uniprot.org, NCBI)

Alternative Names

La-binding protein 1; Rab4-interacting protein; RABIP4FYVE-finger protein EIP1; RUN and FYVE domain containing 1; RUN and FYVE domain-containing 1; RUN and FYVE domain-containing protein 1; ZFYVE12FLJ22251; Zinc finger FYVE domain-containing protein 12 RUFY1 RABIP4, ZFYVE12 RUN and FYVE domain containing 1 RUN and FYVE domain-containing protein 1|FYVE-finger protein EIP1|la-binding protein 1|rab4-interacting protein|zinc finger FYVE domain-containing protein 12

For more info on RUFY1, check out the RUFY1 Infographic

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In this infographic, you will see the following information for RUFY1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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