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- Table of Contents
RUNX2 antibody (M00442) performs well in IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 16 Publications
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Immunofluorescent analysis of Saos-2 cells, using RUNX2 Antibody.
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Anti-RUNX2 Monoclonal Antibody
RUNX2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC, ICC, WB |
Tested Applications
RUNX2 antibody was positively detected by WB in:
human THP-1 whole cell, human U251 whole cell, human RT4 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
RUNX2 antibody was positively detected by IHC in:
human colon tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
RUNX2 antibody was positively detected by ICC/IF in:
Saos-2 cell
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-RUNX2 Monoclonal Antibody
View all RUNX2 Antibodies
View all RUNX2 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
HCO-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human RUNX2.
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
57 kDa
Calculated Molecular Weight
56.6 kDa
Cite This Product
Anti-RUNX2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00442)
Protocols
Western Blot protocol for RUNX2 antibody
Immunohistochemistry (IHC) protocol for RUNX2 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for RUNX2 antibody
Publications
Application:
Species:
miR-33a promotes peri-implantitis by facilitating M1-type polarization of macrophages and inhibiting osteogenesis
Published: 2026-05-04
Journal: ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
Application: WB
Impact Factor: 3.50
Species: Human
PMID: 42092414
Black Phosphorus Nanosheets for Enhanced Osteogenic Differentiation of Dental Follicle Stem Cells
Published: 2026-03-13
Journal: ACS Applied Nano Materials
Application: WB
Impact Factor: 5.50
Species: Human
Injectable, regenerative and anti-infective PEGylated polyglycerol sebacate-modified calcium phosphate cements triggered by berberine and rhBMP-2 for oral bone defect repair
Published: 2026-02-02
Journal: DENTAL MATERIALS
Application: WB
Impact Factor: 6.30
Species: Mouse
PMID: 41633879
Pathological Evidence From an Experimental Rat Model Demonstrates That Aortic Hypoperfusion Contributes to the Development of Medial Arterial Calcification
Published: 2025-12-30
Journal: PATHOLOGY INTERNATIONAL
Application: IF
Impact Factor: 3.40
Species: Rat
PMID: 41467454
Immunomodulatory mesoporous bioactive glass/interleukin-4 nanocomplex promotes bone regeneration
Published: 2025-12-24
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Application: WB,IF
Impact Factor: 8.50
Species: Mouse
PMID: 41453474
Zeolitic imidazolate frameworks exert bioenergetic modulation via the cAMP/PKA/CREB signaling pathway to accelerate periodontal regeneration
Published: 2025-11-27
Journal: CHEMICAL ENGINEERING JOURNAL
Application: WB
Impact Factor: 13.20
Species: Human
Mechanical Stretch Prevents Senescence-Associated Osteogenic Impairment in Mesenchymal Stem Cells via CXCR4/PI3K/AKT Signaling
Published: 2025-10-30
Journal: FASEB JOURNAL
Application: IF,WB
Impact Factor: 4.20
Species: Mouse
PMID: 41165551
Restoration of Cementoblast Mineralization by Glutamine Under Inflammatory Conditions
Published: 2025-10-07
Journal: INTERNATIONAL DENTAL JOURNAL
Application: WB
Impact Factor: 3.70
Species: Mouse
PMID: 41061313
Virulence-Selective Trap–Capture–Kill Antibacterial Nanostructures With Immune–Metabolic Regulation for Treating Implant-Associated Infections
Published: 2025-09-15
Journal: Advanced Science
Application: IF
Impact Factor: 14.10
Species: Rat
PMID: 40952251
Barrier Membrane with Janus Function and Structure for Guided Bone Regeneration
Published: 2024-09-02
Journal: ACS Applied Materials & Interfaces
Application: IF
Impact Factor: 8.30
Species: Mouse
PMID: 39222394
Self-assembled peptide hydrogel loaded with functional peptide dentonin accelerates vascularized bone tissue regeneration in critical-size bone defects
Published: 2024-08-23
Journal: Regenerative Biomaterials
Application: IF
Impact Factor: 5.60
Species: Rat
PMID: 39263324
Network pharmacological study and in vitro studies validation-Molecular dynamics simulation of Cistanche deserticola in promoting periodontitis and bone remodeling
Published: 2024-05-21
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: WB
Impact Factor: 4.80
Species: Mouse
PMID: 38776853
Bisphosphonates activated keratin assembled nanoparticle targeting bone fracture for enhanced immunomodulatory and osteoinductivity
Published: 2024-04-12
Journal: CHEMICAL ENGINEERING JOURNAL
Application: IF
Impact Factor: 13.30
Species: Rat
β-ecdysone promotes osteogenic differentiation of bone marrow mesenchymal stem cells
Published: 2020-04-30
Journal: JOURNAL OF GENE MEDICINE
Application: IHC
Impact Factor: 3.26
Species: Rat
PMID: 32350960
LncRNA expression profiling of BMSCs in osteonecrosis of the femoral head associated with increased adipogenic and decreased osteogenic differentiation
Published: 2018-06-14
Journal: Scientific Reports
Application: IHC
Impact Factor: 4.12
Species: Human
PMID: 29904151
Demineralized bone matrix used for direct pulp capping in rats
Published: 2017-03-02
Journal: PLoS One
Application: IHC
Impact Factor: 2.81
Species: Rat
PMID: 28253279
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Product Promise
We promise Anti-RUNX2 Monoclonal Antibody performs excellently in relevant applications for IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00442
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026