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Product Info Summary
| SKU: | A06438 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-RXFP3/Salpr Antibody
SKU/Catalog Number
A06438
Size
100ul
Form
Liquid
Description
Boster Bio Anti-RXFP3/Salpr Antibody (Catalog# A06438). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RXFP3/Salpr Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06438)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human RXFP3. Immunogen sequence location: 161 - 210
Reactive Species
A06438 is reactive to RXFP3 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
51.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06438 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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Western blot analysis of the lysates from Jurkat cells using RXFP3 antibody.
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Western blot analysis of lysates from K562 cells, using RXFP3 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-RXFP3/Salpr Antibody (A06438)
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Customer Q&As
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3 Customer Q&As for Anti-RXFP3/Salpr Antibody
Question
Is a blocking peptide available for product anti-RXFP3/Salpr antibody (A06438)?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
We do provide the blocking peptide for product anti-RXFP3/Salpr antibody (A06438). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-09-25
Question
Can you help my question with product A06438, anti-RXFP3/Salpr antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A06438 anti-RXFP3/Salpr antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-01
Question
We are currently using anti-RXFP3/Salpr antibody A06438 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2018-07-12
Answer
The anti-RXFP3/Salpr antibody (A06438) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-12
