Anti-RYR2 Antibody Picoband™

Product Name

Anti-RYR2 Antibody Picoband™

View all Ryanodine Receptor 2 Antibodies

SKU/Catalog Number

A00155-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-RYR2 Antibody Picoband™ catalog # A00155-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-RYR2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00155-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human RYR2 recombinant protein (Position: Q1293-T1423).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00155-2 is reactive to RYR2 in Human

Applications

A00155-2 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

564 kDa

Calculated molecular weight

564.567kDa

Background of Ryanodine Receptor 2

Ryanodine receptor 2 (RYR2) is a protein found primarily in cardiac muscle. In humans, it is encoded by the RYR2 gene. This gene encodes a ryanodine receptor found in cardiac muscle sarcoplasmic reticulum. The encoded protein is one of the components of a calcium channel, composed of a tetramer of the ryanodine receptor proteins and a tetramer of FK506 binding protein 1B proteins, that supplies calcium to cardiac muscle. Mutations in this gene are associated with stress-induced polymorphic ventricular tachycardia and arrhythmogenic right ventricular dysplasia.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of RYR2 using anti-RYR2 antibody (A00155-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RYR2 antigen affinity purified polyclonal antibody (Catalog # A00155-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RYR2 at approximately 564 kDa. The expected band size for RYR2 is at 564 kDa.

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Figure 2. IHC analysis of RYR2 using anti-RYR2 antibody (A00155-2).
RYR2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RYR2 Antibody (A00155-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

Gene/Protein Information For RYR2 (Source: Uniprot.org, NCBI)

Alternative Names

ARVC2arrhythmogenic right ventricular dysplasia 2; ARVD2; Cardiac muscle-type ryanodine receptor; hRYR-2; ryanodine receptor 2 (cardiac); ryanodine receptor 2; RyR2; RYR-2; VTSIPCardiac muscle ryanodine receptor-calcium release channel RYR2 ARVC2, ARVD2, RYR-2, RyR, VTSIP ryanodine receptor 2 ryanodine receptor 2|cardiac muscle ryanodine receptor-calcium release channel|cardiac-type ryanodine receptor|islet-type ryanodine receptor|kidney-type ryanodine receptor|ryanodine receptor 2 (cardiac)|type 2 ryanodine receptor

For more info on RYR2, check out the RYR2 Infographic

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In this infographic, you will see the following information for RYR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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