Anti-Protein S100-A5 S100A5 Antibody

S100A5 antibody

Boster Bio Anti-Protein S100-A5 S100A5 Antibody catalog # A10314. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A10314
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-Protein S100-A5 S100A5 Antibody

View all S100A5 Antibodies

SKU/Catalog Number

A10314

Size

100μl

Form

Liquid

Description

Boster Bio Anti-Protein S100-A5 S100A5 Antibody catalog # A10314. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Protein S100-A5 S100A5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10314)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Immunogen

Synthesized peptide derived from internal of human S100A5.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A10314 is reactive to S100A5 in Human, Mouse, Rat

Applications

A10314 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:500-1:3000

Validation Images & Assay Conditions

Gene/Protein Information For S100A5 (Source: Uniprot.org, NCBI)

Uniprot ID

P33763

Gene ID

6276

Gene Name

S100A5

Full Name

Protein S100-A5

Weight

10.744kDa

Superfamily

S-100 family

Alternative Names

Protein S-100D; S100 calcium binding protein A5; S100 calcium-binding protein A5S100Dprotein S100-A5 S100A5 S100D S100 calcium binding protein A5 protein S100-A5

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on S100A5, check out the S100A5 Infographic

S100A5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for S100A5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

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7 Customer Q&As for Anti-Protein S100-A5 S100A5 Antibody

Question

I see that the anti-S100A5 antibody A10314 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-03-13

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-03-13

Question

Does anti-S100A5 antibody A10314 work for WB with kidney?

Verified Customer

Verified customer

Asked: 2019-08-20

Answer

According to the expression profile of kidney, S100A5 is highly expressed in kidney. So, it is likely that anti-S100A5 antibody A10314 will work for WB with kidney.

Boster Scientific Support

Answered: 2019-08-20

Question

Will anti-S100A5 antibody A10314 work on monkey WB with kidney?

Verified Customer

Verified customer

Asked: 2019-05-14

Answer

Our lab technicians have not tested anti-S100A5 antibody A10314 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-S100A5 antibody A10314 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey kidney in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-14

Question

Does A10314 anti-S100A5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-08-08

Answer

You can see on the product datasheet, A10314 anti-S100A5 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-08-08

Question

We are currently using anti-S100A5 antibody A10314 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?

T. Mangal

Verified customer

Asked: 2018-04-20

Answer

The anti-S100A5 antibody (A10314) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-04-20

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-S100A5 antibody A10314. Let me know if you need anything else.

T. Lewis

Verified customer

Asked: 2016-06-28

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-06-28

Question

My question regarding product A10314, anti-S100A5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

T. Wu

Verified customer

Asked: 2014-08-13

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A10314 anti-S100A5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2014-08-13

Size

Total: $299

SKU:A10314

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
A10314
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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