Overview
Product Name |
Anti-SAP102/DLG3 Antibody Picoband™
See more SAP102 products |
Catalog# |
A04152-1 |
Pack Size |
100ug/vial |
Storage & Handling |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Description |
Boster Bio Anti-SAP102/DLG3 Antibody Picoband™ catalog # A04152-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100ug/vial in Lyophilized form antibody. |
Cite This Product |
Anti-SAP102/DLG3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04152-1)
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Antibodies Validation |
Antibodies Validation Information
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Product Specs
Host |
Rabbit |
Reactive Species |
Human, Mouse, Rat |
Applications |
IHC, WB
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
Related Products |
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $100. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round. |
Immunogen |
E. coli-derived human SAP102 recombinant protein (Position: E749-L817). Human SAP102 shares 98.6% amino acid (aa) sequence identity with both mouse and rat SAP102. |
Cross Reactivity |
No cross reactivity with other proteins. |
Gene/Protein Basic Information For DLG3 (Source: Uniprot.org, NCBI)
Uniprot Id | Q92796 |
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NCBI Gene Id | 1741 |
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Species Of This Entry | Human |
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Gene Name | DLG3 |
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Protein Name | Disks large homolog 3 |
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Superfamily | MAGUK family |
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Alternative Names | SAP102|discs, large homolog 3 (Drosophila); KIAA1232discs, large homolog 3 (neuroendocrine-dlg, Drosophila); MRX90MRX; NEDLGdisks large homolog 3; neuroendocrine-DLG; SAP-102; SAP102NE-Dlg; Synapse-associated protein 102; XLMR |
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Molecular Weight | 90314 |
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*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on DLG3, check out the DLG3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for DLG3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the DLG3 infographic
Our Boster Quality Guarantee for Anti-SAP102/DLG3 Antibody Picoband™ covers its use in the following applications.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, Human, By Heat
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human,
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Figure 2. IHC analysis of SAP102 using anti-SAP102 antibody (A04152-1).
SAP102 was detected in paraffin-embedded section of mouse brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SAP102 Antibody (A04152-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of SAP102 using anti-SAP102 antibody (A04152-1).
SAP102 was detected in paraffin-embedded section of rat brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SAP102 Antibody (A04152-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 1. Western blot analysis of SAP102 using anti-SAP102 antibody (A04152-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysate,
Lane 2: mouse brain tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAP102 antigen affinity purified polyclonal antibody (Catalog # A04152-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAP102 at approximately 102KD. The expected band size for SAP102 is at 90KD.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Questions and answers from customers related to A04152-1 Anti-SAP102/DLG3 Antibody Picoband™
1 Related Questions
Question
We are currently using anti-SAP102/DLG3 antibody A04152-1 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
J. Parker
Asked: 2013-05-20
Answer
The anti-SAP102/DLG3 antibody (A04152-1) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-05-20