Anti-SAP102/DLG3 Antibody Picoband™

Product Name

Anti-SAP102/DLG3 Antibody Picoband™

See all SAP102 products

SKU/Catalog Number

A04152-1

Size

100ug/vial

Form

Lyophilized

Description

Boster Bio Anti-SAP102/DLG3 Antibody Picoband™ catalog # A04152-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SAP102/DLG3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04152-1)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E. coli-derived human SAP102 recombinant protein (Position: E749-L817). Human SAP102 shares 98.6% amino acid (aa) sequence identity with both mouse and rat SAP102.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A04152-1 is reactive to DLG3 in Human, Mouse, Rat

Applications

A04152-1 is guaranteed for IHC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, Human, By Heat Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human,

Validation Images & Assay Conditions

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Figure 2. IHC analysis of SAP102 using anti-SAP102 antibody (A04152-1).
SAP102 was detected in paraffin-embedded section of mouse brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SAP102 Antibody (A04152-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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Figure 3. IHC analysis of SAP102 using anti-SAP102 antibody (A04152-1).
SAP102 was detected in paraffin-embedded section of rat brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SAP102 Antibody (A04152-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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Figure 1. Western blot analysis of SAP102 using anti-SAP102 antibody (A04152-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysate,
Lane 2: mouse brain tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAP102 antigen affinity purified polyclonal antibody (Catalog # A04152-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAP102 at approximately 102KD. The expected band size for SAP102 is at 90KD.

Gene/Protein Information For DLG3 (Source: Uniprot.Org, NCBI)

Alternative Names

discs, large homolog 3 (Drosophila); KIAA1232discs, large homolog 3 (neuroendocrine-dlg, Drosophila); MRX90MRX; NEDLGdisks large homolog 3; neuroendocrine-DLG; SAP-102; SAP102NE-Dlg; Synapse-associated protein 102; XLMR DLG3 MRX, MRX90, NEDLG, PPP1R82, SAP102, XLMR discs large MAGUK scaffold protein 3 disks large homolog 3|discs, large homolog 3|neuroendocrine-DLG|protein phosphatase 1, regulatory subunit 82|synapse-associated protein 102

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In this infographic you will see the following information for DLG3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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