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- Table of Contents
SBK1 antibody (A17120-1) performs well in ELISA, Flow Cytometry, IP, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of SH-SY5Y cells using anti-SBK1 antibody (A17120-1).
Overlay histogram showing SH-SY5Y cells stained with A17120-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SBK1 Antibody (A17120-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing SH-SY5Y cells stained with A17120-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SBK1 Antibody (A17120-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-SBK1 Antibody Picoband®
SBK1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IP, IHC, WB |
Tested Applications
SBK1 antibody was positively detected by WB in:
human SH-SY5Y whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue, mouse Neuro-2a whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
SBK1 antibody was positively detected by IHC in:
human lung cancer tissue, human lung cancer tissue, human ovarian cancer tissue, human ovarian cancer tissue, mouse cerebellum tissue, rat cerebellum tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
SBK1 antibody was positively detected by FC in:
SH-SY5Y cell
SBK1 antibody was positively detected by IP in:
MCF-7 whole cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human, Mouse, Rat
Immunoprecipitation
0.5-2 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-SBK1 Antibody Picoband®
View all SBK1 Antibodies
View all SBK1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human SBK1 recombinant protein (Position: E34-Q293). Human SBK1 shares 98.5% amino acid (aa) sequence identity with mouse and rat SBK1.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
46 kDa
Calculated Molecular Weight
46.3 kDa
Background of SBK1
The SBK1 gene, or Serine/threonine-protein kinase SBK1, encodes a protein kinase involved in various cellular processes, including cell cycle regulation, apoptosis, and stress response. SBK1 plays a critical role in signaling pathways governing cell growth and survival. Dysregulation of SBK1 expression or activity has been associated with cancer development and progression, making it a potential therapeutic target in oncology research. Understanding the molecular mechanisms underlying SBK1 function is essential for elucidating its role in both normal cellular physiology and disease states, particularly cancer.
Cite This Product
Anti-SBK1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A17120-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Cow
99%
High Homology
Sheep
99%
High Homology
Dog
99%
High Homology
Mouse
98%
High Homology
Rat
98%
High Homology
Zebrafish
72%
Moderate Homology
Drosophila
37%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for SBK1 antibody
Immunohistochemistry (IHC) protocol for SBK1 antibody
Flow Cytometry (FC) protocol for SBK1 antibody
Immunoprecipitation (IP) protocol for SBK1 antibody
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Product Promise
We promise Anti-SBK1 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IP, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A17120-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 10, 2026