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- Table of Contents
Ly6a antibody (A30403) performs well in Flow Cytometry, IHC, WB and shows reactivity with Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Flow Cytometry analysis of HEPA1-6 cells using anti-Sca1/Ly6A/E antibody (A30403).
Overlay histogram showing HEPA1-6 cells stained with A30403 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Sca1/Ly6A/E Antibody (A30403, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing HEPA1-6 cells stained with A30403 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Sca1/Ly6A/E Antibody (A30403, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Sca1/Ly6A/E Antibody Picoband®
Ly6a antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse | Rabbit | Flow Cytometry, IHC, WB |
Tested Applications
Ly6a antibody was positively detected by WB in:
mouse HEPA1-6 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Ly6a antibody was positively detected by IHC in:
mouse spleen tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Ly6a antibody was positively detected by FC in:
Hepa1-6 cells
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Mouse
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Mouse
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Mouse
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Product Information
Product Name
Anti-Sca1/Ly6A/E Antibody Picoband®
View all LY6A Antibodies
View all LY6A Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of mouse Sca1/Ly6A/E.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
14 kDa
Calculated Molecular Weight
14.4 kDa
Background of Ly6a
Stem cell antigen-1 (Sca-1) is a mouse glycosyl phosphatidylinositol-anchored protein and a cell surface marker found on hematopoietic stem cells (HSCs). It is encoded by the strain-specific Ly-6 E/A allelic gene. Sca-1 has been discovered in several non hematopoietic tissues and can be used to enrich progenitor cell populations other than HSC. It is suggested that Sca-1 could be involved in regulating both B and T cell activation.
Cite This Product
Anti-Sca1/Ly6A/E Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30403)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Ly6a antibody
Immunohistochemistry (IHC) protocol for Ly6a antibody
Flow Cytometry (FC) protocol for Ly6a antibody
Publications
Application:
BMP9-Induced Osteogenetic Differentiation and Bone Formation of Muscle-Derived Stem Cells
PMID: 22319244
Review
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Product Promise
We promise Anti-Sca1/Ly6A/E Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A30403
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 10, 2026