Product Info Summary
SKU: | A01915 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-SCA2/ATXN2 Antibody Picoband™
SKU/Catalog Number
A01915
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SCA2/ATXN2 Antibody Picoband™ catalog # A01915. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SCA2/ATXN2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01915)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SCA2/ATXN2 recombinant protein (Position: Q1278-L1313).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01915 is reactive to ATXN2 in Human
Applications
A01915 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
140-150 kDa
Calculated molecular weight
140.283kDa
Background of Ataxin-2
Ataxin-2, the protein encoded by the ATXN2 gene, contains a polyglutamine tract, long expansions (greater than 33 repeats) of which result in spinocerebellar ataxia-2 (SCA2), an autosomal dominant form of olivopontocerebellar atrophy. The gene for spinocerebellar ataxia type 2 (SCA2) has been mapped to 12q24.1. Ataxin-2 associates with L- and T-plastin and that overexpression of ataxin-2 leads to accumulation of T-plastin in mammalian cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of SCA2/ATXN2 using anti-SCA2/ATXN2 antibody (A01915).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCA2/ATXN2 antigen affinity purified polyclonal antibody (Catalog # A01915) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCA2/ATXN2 at approximately 140-150 kDa. The expected band size for SCA2/ATXN2 is at 140 kDa.
Click image to see more details
Figure 2. IHC analysis of SCA2/ATXN2 using anti-SCA2/ATXN2 antibody (A01915).
SCA2/ATXN2 was detected in a paraffin-embedded section of human thyroiditis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCA2/ATXN2 Antibody (A01915) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of SCA2/ATXN2 using anti-SCA2/ATXN2 antibody (A01915).
SCA2/ATXN2 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCA2/ATXN2 Antibody (A01915) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of SCA2/ATXN2 using anti-SCA2/ATXN2 antibody (A01915).
SCA2/ATXN2 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCA2/ATXN2 Antibody (A01915) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of SCA2/ATXN2 using anti-SCA2/ATXN2 antibody (A01915).
SCA2/ATXN2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCA2/ATXN2 Antibody (A01915) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For ATXN2 (Source: Uniprot.org, NCBI)
Gene Name
ATXN2
Full Name
Ataxin-2
Weight
140.283kDa
Superfamily
ataxin-2 family
Alternative Names
ataxin 2; ataxin 2); ataxin-2; ATX2spinocerebellar ataxia 2 (olivopontocerebellar ataxia 2, autosomal dominant; SCA2FLJ46772; Spinocerebellar ataxia type 2 protein; TNRC13; trinucleotide repeat containing 13; Trinucleotide repeat-containing gene 13 protein ATXN2 ATX2, SCA2, TNRC13 ataxin 2 ataxin-2|spinocerebellar ataxia type 2 protein|trinucleotide repeat-containing gene 13 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATXN2, check out the ATXN2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATXN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SCA2/ATXN2 Antibody Picoband™ (A01915)
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