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Product Info Summary
| SKU: | A16237 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-SCAMP4 Antibody
SKU/Catalog Number
A16237
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SCAMP4 Antibody (Catalog # A16237). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
SCAMP4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-SCAMP4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A16237)
Host
Rabbit
Contents
SCAMP4 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SCAMP4 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human SCAMP4. The immunogen is located within the last 50 amino acids of SCAMP4.
Reactive Species
A16237 is reactive to SCAMP4 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
25.7 kDa
Background of SCAMP4
Secretory carrier membrane proteins (SCAMPs) are integral membrane proteins seen in secretory and endocytic carriers that are thought to play roles in membrane trafficking. One member of this family, SCAMP4, lacks the putative calcium binding and leucine zipper structures as well as the protein-protein interacting NPF domains common to most SCAMP family members. In rats, SCAMP4 mRNA is repressed by progesterone in brain regions associated with female sexual behavior. At least three isoforms of SCAMP4 are known to exist. This SCAMP4 antibody is predicted to not cross-react with any other member of the secretory carrier membrane protein family.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A16237 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SCAMP4 antibody can be used for detection of SCAMP4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SCAMP4 in 3T3 cell lysate with SCAMP4 antibody at (A) 1 and (B) 2 μg/mL.
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Immunofluorescence of SCAMP4 in Human Brain cells with SCAMP4 antibody at 20 μg/mL.
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Immunohistochemistry of SCAMP4 in human brain tissue with SCAMP4 antibody at 2.5 μg/mL.
Specific Publications For Anti-SCAMP4 Antibody (A16237)
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